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product-image-AAA292225_WB13.jpg WB (Western Blot) (Sample:B16 Cell (Mouse) Lysate at 40 ugDU145 Cell (Human) Lysate at 40 ugPrimary: Anti-EGFR5 (bs-9813R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 49 kDObserved band size: 53 kD)

Rabbit anti-Rat EGFR5 Polyclonal Antibody | anti-EGFR5 antibody

EGFR5 Polyclonal Antibody

Gene Names
CLEC14A; CEG1; EGFR-5; C14orf27
Reactivity
Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
EGFR5, Antibody; EGFR5 Polyclonal Antibody; C-type lectin domain family 14 member A; Epidermal growth factor receptor 5; EGFR-5; CLEC14A; C14orf27; EGFR5; anti-EGFR5 antibody
Ordering
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-EGFR5 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Sample:B16 Cell (Mouse) Lysate at 40 ugDU145 Cell (Human) Lysate at 40 ugPrimary: Anti-EGFR5 (bs-9813R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 49 kDObserved band size: 53 kD)

product-image-AAA292225_WB13.jpg WB (Western Blot) (Sample:B16 Cell (Mouse) Lysate at 40 ugDU145 Cell (Human) Lysate at 40 ugPrimary: Anti-EGFR5 (bs-9813R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 49 kDObserved band size: 53 kD)

FCM/FACS (Flow Cytometry)

(Blank control(blue):293T (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-EGFR5 antibody(bs-9813R), Dilution: 1 ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.)

product-image-AAA292225_FCM15.jpg FCM/FACS (Flow Cytometry) (Blank control(blue):293T (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-EGFR5 antibody(bs-9813R), Dilution: 1 ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,636 Da
NCBI Official Full Name
C-type lectin domain family 14 member A
NCBI Official Synonym Full Names
C-type lectin domain family 14, member A
NCBI Official Symbol
CLEC14A
NCBI Official Synonym Symbols
CEG1; EGFR-5; C14orf27
NCBI Protein Information
C-type lectin domain family 14 member A; epidermal growth factor receptor 5; ClECT and EGF-like domain containing protein
UniProt Protein Name
C-type lectin domain family 14 member A
UniProt Gene Name
CLEC14A
UniProt Synonym Gene Names
C14orf27; EGFR5; EGFR-5
UniProt Entry Name
CLC14_HUMAN

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Product Notes

The EGFR5 clec14a (Catalog #AAA292225) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EGFR5 Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EGFR5 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the EGFR5 clec14a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFR5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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