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IHC (Immunohistchemistry)
(EHHADH/Enoyl-Coa Hydratase Antibody-Formalin-fixed and paraffin-embedded human hepatocarcinoma with EHHADH Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit anti-Mouse, Human EHHADH/Enoyl-Coa Hydratase Polyclonal Antibody | anti-EHHADH antibody
EHHADH/Enoyl-Coa Hydratase Rabbit anti-Human Polyclonal (aa662-690) Antibody
Gene Names
EHHADH; LBP; ECHD; LBFP; PBFE; FRTS3; L-PBE
Reactivity
Mouse, Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Protein A purified
Synonyms
EHHADH/Enoyl-Coa Hydratase, Antibody; EHHADH/Enoyl-Coa Hydratase Rabbit anti-Human Polyclonal (aa662-690) Antibody; IHC-plus EHHADH/Enoyl-Coa Hydratase Antibody (aa662-690); EHHADH; 3;2-trans-enoyl-CoA isomerase; ECHD; LBFP; L-PBE; Enoyl-coa hydratase; PBE; PBFE; anti-EHHADH antibody
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This EHHADH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 662-690 amino acids from the C-terminal region of human EHHADH.
Purity/Purification
Protein A purified
Form/Format
PBS, 0.09% Sodium Azide
Applicable Applications for anti-EHHADH antibody
Flow Cytometry (FC/FACS), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
Target
Human EHHADH/Enoyl-Coa Hydratase
Conjugation
Unconjugated
Epitope
aa662-690
Preparation and Storage
Maintain refrigerated at 2 degree C to 8 degree C for up to 2 weeks. For long term storage aliquot and store at -20 degree C. Avoid freeze-thaw cycles.
IHC (Immunohistchemistry)
(EHHADH/Enoyl-Coa Hydratase Antibody-Formalin-fixed and paraffin-embedded human hepatocarcinoma with EHHADH Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistchemistry)
(EHHADH/Enoyl-Coa Hydratase Antibody-Formalin-fixed and paraffin-embedded human hepatocarcinoma with EHHADH Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(EHHADH/Enoyl-Coa Hydratase Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(EHHADH/Enoyl-Coa Hydratase Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(EHHADH/Enoyl-Coa Hydratase Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(EHHADH/Enoyl-Coa Hydratase Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(EHHADH/Enoyl-Coa Hydratase Antibody-Western blot of lysates from mouse kidney, rat kidney and liver tissue (from left to right), using EHHADH Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(EHHADH/Enoyl-Coa Hydratase Antibody-Western blot of lysates from mouse kidney, rat kidney and liver tissue (from left to right), using EHHADH Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(EHHADH/Enoyl-Coa Hydratase Antibody-Western blot of EHHADH Antibody in mouse liver (lane 1), kidney(lane 2) tissue lysates (35 ug/lane). EHHADH (arrow) was detected using the purified antibody.)
WB (Western Blot)
(EHHADH/Enoyl-Coa Hydratase Antibody-Western blot of EHHADH Antibody in mouse liver (lane 1), kidney(lane 2) tissue lysates (35 ug/lane). EHHADH (arrow) was detected using the purified antibody.)
Application Data
(EHHADH/Enoyl-Coa Hydratase Antibody-Flow cytometric of HepG2 cells using EHHADH Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Application Data
(EHHADH/Enoyl-Coa Hydratase Antibody-Flow cytometric of HepG2 cells using EHHADH Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-EHHADH antibody
Enoyl-Coa Hydratase antibody is an unconjugated rabbit polyclonal antibody to Enoyl-Coa Hydratase (EHHADH)(aa662-690) from human. It is reactive with human and mouse. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,154 Da
NCBI Official Full Name
peroxisomal bifunctional enzyme isoform 1
NCBI Official Synonym Full Names
enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase
NCBI Official Symbol
EHHADH
NCBI Official Synonym Symbols
LBP; ECHD; LBFP; PBFE; FRTS3; L-PBE
NCBI Protein Information
peroxisomal bifunctional enzyme
UniProt Protein Name
Peroxisomal bifunctional enzyme
UniProt Gene Name
EHHADH
UniProt Synonym Gene Names
ECHD; PBE; PBFE
UniProt Entry Name
ECHP_HUMAN
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Product Notes
The EHHADH ehhadh (Catalog #AAA21380) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EHHADH/Enoyl-Coa Hydratase Rabbit anti-Human Polyclonal (aa662-690) Antibody reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's EHHADH/Enoyl-Coa Hydratase can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB). Researchers should empirically determine the suitability of the EHHADH ehhadh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EHHADH/Enoyl-Coa Hydratase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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