WB (Western Blot)
(Western blot analysis of extracts from 293, using EID1 Antibody. Lane 1 was treated with the antigen-specific peptide.)
Rabbit anti-Human EID1 Polyclonal Antibody | anti-EID1 antibody
EID1 Antibody
Gene Names
EID1; CRI1; EID-1; RBP21; PTD014; C15orf3; PNAS-22; IRO45620
Reactivity
Human
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
EID1, Antibody; EID1 Antibody; 21 kDa pRb associated protein; 21 kDa pRb-associated protein; C15orf3; CBP; Chromosome 15 open reading frame 3; CREBBP/EP300 inhibitor 1; CREBBP/EP300 inhibitory protein 1; CRI 1; CRI1; E1A like inhibitor of differentiation; E1A-like inhibitor of differentiation 1; EID 1; EID-1; EID1; EID1_HUMAN; EP300 interacting inhibitor of differentiation 1; EP300-interacting inhibitor of differentiation 1; IRO45620; MGC138883; MGC138884; NB4 apoptosis related protein; PNAS 22; PNAS22; PTD014; Rb and p300 binding protein EID 1; Rb and p300 binding protein EID1; RBP21; Retinoblastoma protein associated protein; anti-EID1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
EID1 antibody detects endogenous levels of total EID1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
187
Applicable Applications for anti-EID1 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Nucleus. Cytoplasm. May shuttle between nucleus and cytoplasm.
Tissue Specificity
Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A-549 and various leukemia cell lines.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Sheep (100%), Rabbit (91%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from 293, using EID1 Antibody. Lane 1 was treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of extracts from 293, using EID1 Antibody. Lane 1 was treated with the antigen-specific peptide.)
IF (Immunofluorescence)
(AAA324179 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA324179 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells, using EID1 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells, using EID1 antibody.)
Related Product Information for anti-EID1 antibody
Function: Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.
Subunit Structure: Interacts via its LXCXE motif with the entire pocket region of RB1. Interacts with EP300, NR0B2 and TRIM27.
Post-translational Modifications: Ubiquitinated in U2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.
Subunit Structure: Interacts via its LXCXE motif with the entire pocket region of RB1. Interacts with EP300, NR0B2 and TRIM27.
Post-translational Modifications: Ubiquitinated in U2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 21 kD
Predicted: 21 kDa
Predicted: 21 kDa
NCBI Official Full Name
EP300-interacting inhibitor of differentiation 1
NCBI Official Synonym Full Names
EP300 interacting inhibitor of differentiation 1
NCBI Official Symbol
EID1
NCBI Official Synonym Symbols
CRI1; EID-1; RBP21; PTD014; C15orf3; PNAS-22; IRO45620
NCBI Protein Information
EP300-interacting inhibitor of differentiation 1
UniProt Protein Name
EP300-interacting inhibitor of differentiation 1
UniProt Gene Name
EID1
UniProt Synonym Gene Names
EID-1
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Product Notes
The EID1 eid1 (Catalog #AAA324179) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EID1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EID1 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the EID1 eid1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EID1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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