WB (Western Blot)
(Western blot analysis of EIF2AK2 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Rabbit anti EIF2AK2 antibody (AAA50453) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 184 second and (B) 30 second exposure.)
Rabbit anti-Human EIF2AK2 Polyclonal Antibody | anti-EIF2AK2 antibody
EIF2AK2 antibody
Gene Names
EIF2AK2; PKR; PRKR; EIF2AK1
Reactivity
Human
Applications
Western Blot
Purity
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Synonyms
EIF2AK2, Antibody; EIF2AK2 antibody; RABBIT ANTI EIF2AK2; anti-EIF2AK2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Form/Format
Purified IgG - liquid
Phosphate buffered saline
0.09% Sodium Azide, <50% Glycerol
Phosphate buffered saline
0.09% Sodium Azide, <50% Glycerol
Concentration
IgG concentration 0.5mg/ml (varies by lot)
Applicable Applications for anti-EIF2AK2 antibody
WB (Western Blot)
Immunogen
Recombinant protein of human EIF2AK2
Preparation and Storage
Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles
12 months from date of despatch.
12 months from date of despatch.
WB (Western Blot)
(Western blot analysis of EIF2AK2 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Rabbit anti EIF2AK2 antibody (AAA50453) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 184 second and (B) 30 second exposure.)
WB (Western Blot)
(Western blot analysis of EIF2AK2 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Rabbit anti EIF2AK2 antibody (AAA50453) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 184 second and (B) 30 second exposure.)
WB (Western Blot)
(Western blot analysis of whole cell lysates probed with EIF2AK2 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000) and visualized on the ChemiDoc MP with 16 second exposure. Arrow points to EIF2AK2 (molecular weight 74 kDa))
WB (Western Blot)
(Western blot analysis of whole cell lysates probed with EIF2AK2 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000) and visualized on the ChemiDoc MP with 16 second exposure. Arrow points to EIF2AK2 (molecular weight 74 kDa))
Related Product Information for anti-EIF2AK2 antibody
Rabbit anti Human EIF2AK2 antibody recognizes EIF2AK2, also known as P1/eIF-2A protein kinase, double stranded RNA activated protein kinase, eIF-2A protein kinase 2, interferon-induced double-stranded RNA-activated protein kinase, interferon-inducible elF2alpha kinase and tyrosine-protein kinase EIF2AK2.
The protein encoded by EIF2AK2 is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for EIF2AK2 (provided by RefSeq, Oct 2011).
Rabbit anti Human EIF2AK2 detects a band of 74 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by EIF2AK2 is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for EIF2AK2 (provided by RefSeq, Oct 2011).
Rabbit anti Human EIF2AK2 detects a band of 74 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,094 Da
NCBI Official Full Name
interferon-induced, double-stranded RNA-activated protein kinase isoform a
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2-alpha kinase 2
NCBI Official Symbol
EIF2AK2
NCBI Official Synonym Symbols
PKR; PRKR; EIF2AK1
NCBI Protein Information
interferon-induced, double-stranded RNA-activated protein kinase; p68 kinase; eIF-2A protein kinase 2; P1/eIF-2A protein kinase; tyrosine-protein kinase EIF2AK2; interferon-inducible elF2alpha kinase; double stranded RNA activated protein kinase; protein
UniProt Protein Name
Interferon-induced, double-stranded RNA-activated protein kinase
UniProt Gene Name
EIF2AK2
UniProt Synonym Gene Names
PKR; PRKR; eIF-2A protein kinase 2; PKR
UniProt Entry Name
E2AK2_HUMAN
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Product Notes
The EIF2AK2 eif2ak2 (Catalog #AAA50453) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF2AK2 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF2AK2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the EIF2AK2 eif2ak2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF2AK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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