IF (Immunofluorescence)
(AAA325220 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA325220) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
Rabbit EIF2B4 Polyclonal Antibody | anti-EIF2B4 antibody
EIF2B4 Antibody
Gene Names
EIF2B4; EIF2B; EIF-2B; EIF2Bdelta
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
EIF2B4, Antibody; EIF2B4 Antibody; EI2BD_HUMAN; EIF 2B; eIF 2B GDP GTP exchange factor subunit delta; eIF-2B GDP-GTP exchange factor subunit delta; Eif2b4; EIF2Bdelta; eukaryotic translation initiation factor 2B subunit 4; Translation initiation factor eIF-2B subunit delta; anti-EIF2B4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
EIF2B4 antibody detects endogenous levels of total EIF2B4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
523
Applicable Applications for anti-EIF2B4 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human EIF2B4,corresponding to a region within the internal amino acids.
Function
Catalyzes the exchange of eukartotic intiation factor 2-bound GDP for GTP.
Similarity
Belongs to the eIF-2B alpha/beta/delta subunits family.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA325220 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA325220) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA325220 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA325220) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA325220 staining LOVO cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA325220 staining LOVO cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells using EIF2B4 antibody.The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells using EIF2B4 antibody.The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-EIF2B4 antibody
Function: Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
Subunit Structure: Complex of five different subunits; alpha, beta, gamma, delta and epsilon.
Similarity: Belongs to the eIF-2B alpha/beta/delta subunits family.
Subunit Structure: Complex of five different subunits; alpha, beta, gamma, delta and epsilon.
Similarity: Belongs to the eIF-2B alpha/beta/delta subunits family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57 KD
NCBI Official Full Name
translation initiation factor eIF-2B subunit delta isoform 2
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2B subunit delta
NCBI Official Symbol
EIF2B4
NCBI Official Synonym Symbols
EIF2B; EIF-2B; EIF2Bdelta
NCBI Protein Information
translation initiation factor eIF-2B subunit delta
UniProt Protein Name
Translation initiation factor eIF-2B subunit delta
UniProt Gene Name
EIF2B4
UniProt Synonym Gene Names
EIF2BD
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Product Notes
The EIF2B4 eif2b4 (Catalog #AAA325220) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF2B4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EIF2B4 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the EIF2B4 eif2b4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF2B4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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