WB (Western Blot)
(Western blot analysis of EIF2C3 using COLO205 whole cell lysates)
Rabbit EIF2C3 Polyclonal Antibody | anti-EIF2C3 antibody
EIF2C3 Antibody
Gene Names
AGO3; EIF2C3
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
EIF2C3, Antibody; EIF2C3 Antibody; 5730550L01Rik; Ago 3; Ago3; AGO3_HUMAN; argonaute 3; Argonaute3; eIF 2C 3; eIF-2C 3; eIF2C 3; Eif2c3; EIF2C3 protein; Eukaryotic translation initiation factor 2C 3; Eukaryotic translation initiation factor 2C3; FLJ12765; hAgo3; MGC86946; Protein argonaute-3; anti-EIF2C3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
EIF2C3 antibody detects endogenous levels of total EIF2C3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
860
Applicable Applications for anti-EIF2C3 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human EIF2C3
Predicted Cross Reactivity
Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken
Similarity
Zebrafish (83%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of EIF2C3 using COLO205 whole cell lysates)
WB (Western Blot)
(Western blot analysis of EIF2C3 using COLO205 whole cell lysates)
IHC (Immunohistochemistry)
(AAA327982 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA327982 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-EIF2C3 antibody
Function: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4.
Subunit Structure: Interacts with EIF4B, IMP8, PRMT5 and TNRC6B. Interacts with APOBEC3F, APOBEC3G and APOBEC3H. Interacts with EDC4.
Similarity: Belongs to the argonaute family. Ago subfamily.
Subunit Structure: Interacts with EIF4B, IMP8, PRMT5 and TNRC6B. Interacts with APOBEC3F, APOBEC3G and APOBEC3H. Interacts with EDC4.
Similarity: Belongs to the argonaute family. Ago subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 97 kDa
Predicted: 98 kDa
Predicted: 98 kDa
NCBI Official Full Name
protein argonaute-3 isoform a
NCBI Official Synonym Full Names
argonaute 3, RISC catalytic component
NCBI Official Symbol
AGO3
NCBI Official Synonym Symbols
EIF2C3
NCBI Protein Information
protein argonaute-3
UniProt Protein Name
Protein argonaute-3
UniProt Gene Name
AGO3
UniProt Synonym Gene Names
EIF2C3; Argonaute3
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Product Notes
The EIF2C3 ago3 (Catalog #AAA327982) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF2C3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EIF2C3 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the EIF2C3 ago3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF2C3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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