Rabbit EIF3CL Polyclonal Antibody | anti-EIF3C antibody

EIF3CL Antibody (N-term)

Cat. Num. AAA286077

• Gene Names: EIF3C; EIF3CL; EIF3S8; eIF3-p110
• Reactivity: Human; (Predicted: Bovine, Mouse, Rat)
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: EIF3CL, Antibody; EIF3CL Antibody (N-term); Eukaryotic translation initiation factor 3 subunit C {ECO:0000255|HAMAP-Rule:MF_03002}; eIF3c {ECO:0000255|HAMAP-Rule:MF_03002}; Eukaryotic translation initiation factor 3 subunit 8 {ECO:0000255|HAMAP-Rule:MF_03002}; eIF3 p110 {ECO:0000255|HAMAP-Rule:MF_03002}; EIF3C {ECO:0000255|HAMAP-Rule:MF_03002}; anti-EIF3C antibody

• Host: Rabbit
• Reactivity: Human; (Predicted: Bovine, Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This EIF3CL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-155 amino acids from the N-terminal region of human EIF3CL.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 127-155

• Applicable Applications for anti-EIF3C antibody: ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
• Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed: , PubMed:25849773, PubMed: ). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl- tRNAi and eIF-5 to form the 43S pre-initiation complex (43SPIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed: ).
• Cellular Location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03002}.

FCM/FACS (Flow Cytometry)

(EIF3CL Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(EIF3CL Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF3CL Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(All lanes : Anti-EIF3CL Antibody (N-term) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 105 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-EIF3C antibody

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

References

Imielinski, M., et al. Nat. Genet. 41(12):1335-1340(2009)
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008)
Masutani, M., et al. EMBO J. 26(14):3373-3383(2007)
Damoc, E., et al. Mol. Cell Proteomics 6(7):1135-1146(2007)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 83700233
• NCBI GeneID: 8663
• NCBI Accession #: NP_001032897.1; NP_001186071.1; NP_001254503.1; NP_003743.1
• NCBI GenBank Nucleotide #: NM_001037808.2
• UniProt Accession #: Q99613; O00215; Q9BW98; A8K7Z0; B2RXG3; B4E1D5; H3BRV0
• Molecular Weight: 105344
• NCBI Official Full Name: eukaryotic translation initiation factor 3 subunit C isoform a
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 3, subunit C
• NCBI Official Symbol: EIF3C
• NCBI Official Synonym Symbols: EIF3CL; EIF3S8; eIF3-p110
• NCBI Protein Information: eukaryotic translation initiation factor 3 subunit C
• UniProt Protein Name: Eukaryotic translation initiation factor 3 subunit C
• UniProt Gene Name: EIF3C
• UniProt Synonym Gene Names: eIF3c
• UniProt Entry Name: EIF3C_HUMAN