Rabbit EIF3G Polyclonal Antibody | anti-EIF3G antibody

EIF3G Antibody (Center)

Cat. Num. AAA287304

• Gene Names: EIF3G; EIF3S4; EIF3-P42; eIF3-p44; eIF3-delta
• Reactivity: Human (Predicted Reactivity: Xenopus, Bovine, Zebrafish, Mouse, Rat)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: EIF3G, Antibody; EIF3G Antibody (Center); Eukaryotic translation initiation factor 3 subunit G {ECO:0000255|HAMAP-Rule:MF_03006}; eIF3g {ECO:0000255|HAMAP-Rule:MF_03006}; Eukaryotic translation initiation factor 3 RNA-binding subunit {ECO:0000255|HAMAP-Rule:MF_03006}; eIF-3 RNA-binding subunit {ECO:0000255|HAMAP-Rule:MF_03006}; Eukaryotic translation initiation factor 3 subunit 4 {ECO:0000255|HAMAP-Rule:MF_03006}; eIF-3-delta {ECO:0000255|HAMAP-Rule:MF_03006}; eIF3 p42 {ECO:0000255|HAMAP-Rule:MF_03006}; eIF3 p44 {ECO:0000255|HAMAP-Rule:MF_03006}; EIF3G {ECO:0000255|HAMAP-Rule:MF_03006}; anti-EIF3G antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Xenopus, Bovine, Zebrafish, Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This EIF3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-238 amino acids from the Central region of human EIF3G.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 209-238
• Sequence Length: 320

• Applicable Applications for anti-EIF3G antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(EIF3G Antibody (Center) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(EIF3G Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EIF3G Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(EIF3G Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF3G antibody detected the EIF3G protein (arrow).)

Related Product Information for anti-EIF3G antibody

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.

References

Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008)
Masutani, M., et al. EMBO J. 26(14):3373-3383(2007)
Damoc, E., et al. Mol. Cell Proteomics 6(7):1135-1146(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 49472822
• NCBI GeneID: 8666
• NCBI Accession #: NP_003746.2
• NCBI GenBank Nucleotide #: NM_003755.3
• UniProt Accession #: O75821; O14801; Q969U5
• Molecular Weight: 35611
• NCBI Official Full Name: eukaryotic translation initiation factor 3 subunit G
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 3, subunit G
• NCBI Official Symbol: EIF3G
• NCBI Official Synonym Symbols: EIF3S4; EIF3-P42; eIF3-p44; eIF3-delta
• NCBI Protein Information: eukaryotic translation initiation factor 3 subunit G
• UniProt Protein Name: Eukaryotic translation initiation factor 3 subunit G
• UniProt Gene Name: EIF3G
• UniProt Synonym Gene Names: eIF3g; eIF-3 RNA-binding subunit
• UniProt Entry Name: EIF3G_HUMAN