FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of hela cells using EIF3H Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit EIF3H Polyclonal Antibody | anti-EIF3H antibody
EIF3H Antibody (C-term)
Gene Names
EIF3H; EIF3S3; eIF3-p40; eIF3-gamma
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Chicken, Xenopus)
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
EIF3H, Antibody; EIF3H Antibody (C-term); Eukaryotic translation initiation factor 3 subunit H {ECO:0000255|HAMAP-Rule:MF_03007}; eIF3h {ECO:0000255|HAMAP-Rule:MF_03007}; Eukaryotic translation initiation factor 3 subunit 3 {ECO:0000255|HAMAP-Rule:MF_03007}; eIF-3-gamma; eIF3 p40 subunit {ECO:0000255|HAMAP-Rule:MF_03007}; EIF3H {ECO:0000255|HAMAP-Rule:MF_03007}; anti-EIF3H antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Chicken, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EIF3H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-263 amino acids from the C-terminal region of human EIF3H.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
234-263
Sequence Length
352
Applicable Applications for anti-EIF3H antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of hela cells using EIF3H Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of hela cells using EIF3H Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with EIF3H Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with EIF3H Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(EIF3H Antibody (N-term) western blot analysis in Hela cell line and mouse bladder tissue lysates (35ug/lane).This demonstrates the EIF3H antibody detected the EIF3H protein (arrow).)
WB (Western Blot)
(EIF3H Antibody (N-term) western blot analysis in Hela cell line and mouse bladder tissue lysates (35ug/lane).This demonstrates the EIF3H antibody detected the EIF3H protein (arrow).)
Related Product Information for anti-EIF3H antibody
EIF3H is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Product Categories/Family for anti-EIF3H antibody
References
Cappuzzo,F., J Thorac Oncol 4 (4), 472-478 (2009)
Zhou,M., Proc. Natl. Acad. Sci. U.S.A. 105 (47), 18139-18144 (2008)
Zhou,M., Proc. Natl. Acad. Sci. U.S.A. 105 (47), 18139-18144 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39930
NCBI Official Full Name
eukaryotic translation initiation factor 3 subunit H
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 3, subunit H
NCBI Official Symbol
EIF3H
NCBI Official Synonym Symbols
EIF3S3; eIF3-p40; eIF3-gamma
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit H
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit H
UniProt Gene Name
EIF3H
UniProt Synonym Gene Names
eIF3h
UniProt Entry Name
EIF3H_HUMAN
Similar Products
Product Notes
The EIF3H eif3h (Catalog #AAA286544) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 234-263. The EIF3H Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Bovine, Chicken, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's EIF3H can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the EIF3H eif3h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF3H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.