WB (Western Blot)
(Detection of human eIF3J/EIF3S1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-eIF3J/EIF3S1 antibody AAA211614 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. eIF3J/EIF3S1 was also immunoprecipitated by rabbit anti-eIF3J/EIF3S1 antibody BL6675, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
Rabbit anti-Human, Mouse eIF3J/EIF3S1 Polyclonal Antibody | anti-eIF3J/EIF3S1 antibody
Rabbit anti-eIF3J/EIF3S1 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
eIF3J/EIF3S1, Antibody; Rabbit anti-eIF3J/EIF3S1 Antibody, Affinity Purified; eIF3j; eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD); eukaryotic translation initiation factor 3 subunit J; Eukaryotic translation initiation factor 3 subunit 1; eIF3-p35; eIF3-alpha; eIF-3-alpha; eIF3 p35; EIF3S1; Eukaryotic translation initiation factor 3 subunit J; anti-eIF3J/EIF3S1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-eIF3J/EIF3S1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Orangutan
Immunogen
Between 208 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human eIF3J/EIF3S1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-eIF3J/EIF3S1 antibody AAA211614 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. eIF3J/EIF3S1 was also immunoprecipitated by rabbit anti-eIF3J/EIF3S1 antibody BL6675, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
WB (Western Blot)
(Detection of human eIF3J/EIF3S1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-eIF3J/EIF3S1 antibody AAA211614 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. eIF3J/EIF3S1 was also immunoprecipitated by rabbit anti-eIF3J/EIF3S1 antibody BL6675, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human and mouse eIF3J/EIF3S1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eIF3J/EIF3S1 (Cat. No. AAA211614) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human and mouse eIF3J/EIF3S1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eIF3J/EIF3S1 (Cat. No. AAA211614) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-eIF3J/EIF3S1 antibody
Eukaryotic initiation factor 3 subunit J (eIF3J) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end of cellular mRNAs near the cap structure (m7GpppN). eIF3J is proposed to act as a factor that further stabilizes the interaction between eIF3 and the ribosome.
Product Categories/Family for anti-eIF3J/EIF3S1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The eIF3J/EIF3S1 eif3j (Catalog #AAA211614) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-eIF3J/EIF3S1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's eIF3J/EIF3S1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the eIF3J/EIF3S1 eif3j for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF3J/EIF3S1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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