WB (Western Blot)
(Detection of human and mouse EIF3L by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EIF3L antibody AAA212913 (lot AAA212913-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse EIF3L Polyclonal Antibody | anti-EIF3L antibody
Rabbit anti-EIF3L Antibody, Affinity Purified
Gene Names
EIF3L; EIF3EIP; EIF3S11; HSPC021; HSPC025; MSTP005; EIF3S6IP
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
EIF3L, Antibody; Rabbit anti-EIF3L Antibody, Affinity Purified; eukaryotic translation initiation factor 3 subunit E-interacting protein; MSTP005; HSPC025; HSPC021; eukaryotic translation initiation factor 3 subunit L; EIF3S6IP; EIF3S11; eIF3l; EIF3EIP; eIEF associated protein HSPC021; eukaryotic translation initiation factor 3 subunit 6-interacting protein; Eukaryotic translation initiation factor 3 subunit L; anti-EIF3L antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-EIF3L antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Chicken, Bovine, Chimpanzee
Immunogen
Between 514 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse EIF3L by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EIF3L antibody AAA212913 (lot AAA212913-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse EIF3L by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EIF3L antibody AAA212913 (lot AAA212913-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human EIF3L by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EIF3L antibody AAA212913 (lot AAA212913-1) used for IP at 6 ug per reaction. EIF3L was also immunoprecipitated by rabbit anti-EIF3L antibody For blotting immunoprecipitated EIF3L, AAA212913 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human EIF3L by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EIF3L antibody AAA212913 (lot AAA212913-1) used for IP at 6 ug per reaction. EIF3L was also immunoprecipitated by rabbit anti-EIF3L antibody For blotting immunoprecipitated EIF3L, AAA212913 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-EIF3L antibody
Eukaryotic translation initiation factor 3 subunit L (EIF3L) is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y262].
Product Categories/Family for anti-EIF3L antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,014 Da
NCBI Official Full Name
eukaryotic translation initiation factor 3 subunit L isoform 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 3 subunit L
NCBI Official Symbol
EIF3L
NCBI Official Synonym Symbols
EIF3EIP; EIF3S11; HSPC021; HSPC025; MSTP005; EIF3S6IP
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit L
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit L
UniProt Gene Name
EIF3L
UniProt Entry Name
EIF3L_HUMAN
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Product Notes
The EIF3L eif3l (Catalog #AAA212913) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-EIF3L Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EIF3L can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the EIF3L eif3l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF3L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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