WB (Western Blot)
(Detection of human and mouse EIF3M by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EIF3M antibody AAA212918 (lot AAA212918-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Rabbit anti-Human, Mouse EIF3M Polyclonal Antibody | anti-EIF3M antibody
Rabbit anti-EIF3M Antibody, Affinity Purified
Gene Names
EIF3M; B5; GA17; PCID1; TANGO7; hfl-B5
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
EIF3M, Antibody; Rabbit anti-EIF3M Antibody, Affinity Purified; GA17; transport and golgi organization 7 homolog; TANGO7; PCID1; PCI domain-containing protein 1; B5; hfl-B5; fetal lung protein B5; eukaryotic translation initiation factor 3 subunit M; eIF3m; dendritic cell protein; B5 receptor; PCI domain containing 1 (herpesvirus entry mediator); Eukaryotic translation initiation factor 3 subunit M; anti-EIF3M antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-EIF3M antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine, Orangutan
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse EIF3M by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EIF3M antibody AAA212918 (lot AAA212918-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
WB (Western Blot)
(Detection of human and mouse EIF3M by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EIF3M antibody AAA212918 (lot AAA212918-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human EIF3M by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EIF3M antibody AAA212918 (lot AAA212918-1) used for IP at 6 ug per reaction. EIF3M was also immunoprecipitated by rabbit anti-EIF3M antibody BL18577. For blotting immunoprecipitated EIF3M, AAA212918 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human EIF3M by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EIF3M antibody AAA212918 (lot AAA212918-1) used for IP at 6 ug per reaction. EIF3M was also immunoprecipitated by rabbit anti-EIF3M antibody BL18577. For blotting immunoprecipitated EIF3M, AAA212918 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Related Product Information for anti-EIF3M antibody
EIF3M is part of the eurkaryotic translation initiation factor 3 complete (eIF-3) required for protein synthesis. Elevated levels of EIF3M are present in cancer cell lines. Inactivation of EIF3M has been shown to interfere with translation of herpes virus mRNAs by preventing the association of mRNAs with the ribosomes [taken from NCBI Entrez Gene (Gene ID: 10480)].
Product Categories/Family for anti-EIF3M antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,865 Da
NCBI Official Full Name
eukaryotic translation initiation factor 3 subunit M
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 3, subunit M
NCBI Official Symbol
EIF3M
NCBI Official Synonym Symbols
B5; GA17; PCID1; TANGO7; hfl-B5
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit M; EIF3M; B5 receptor; fetal lung protein B5; dendritic cell protein; PCI domain-containing protein 1; transport and golgi organization 7 homolog; PCI domain containing 1 (herpesvirus entry mediator)
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit M
UniProt Gene Name
EIF3M
UniProt Synonym Gene Names
; hFL-B5
UniProt Entry Name
EIF3M_HUMAN
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Product Notes
The EIF3M eif3m (Catalog #AAA212918) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-EIF3M Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EIF3M can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the EIF3M eif3m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF3M, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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