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WB (Western Blot)
(Detection of human and mouse eIF4AIII/EIF4A3 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibody AAA212091 (lot AAA212091-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse eIF4AIII/EIF4A3 Polyclonal Antibody | anti-eIF4AIII/EIF4A3 antibody
Rabbit anti-eIF4AIII/EIF4A3 Antibody, Affinity Purified
Gene Names
EIF4A3; RCPS; DDX48; MUK34; NUK34; NMP265; eIF4AIII
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
eIF4AIII/EIF4A3, Antibody; Rabbit anti-eIF4AIII/EIF4A3 Antibody, Affinity Purified; NMP 265; RCPS; NUK34; NMP265; MUK34; hNMP 265; Fal1; Eukaryotic translation initiation factor 4A isoform 3; eukaryotic translation initiation factor 4A; eukaryotic initiation factor 4A-III; eIF4A-III; eIF-4A-III; eIF4AIII; DEAD box protein 48; ATP-dependent RNA helicase DDX48; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; nuclear matrix protein 265; DDX48; ATP-dependent RNA helicase eIF4A-3; eukaryotic initiation factor 4A-like NUK-34; Eukaryotic initiation factor 4A-III; eukaryotic translation initiation factor 4A3; anti-eIF4AIII/EIF4A3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-eIF4AIII/EIF4A3 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Zebrafish, X. laevis, X. tropicalis, Chicken, Bovine, Pig
Immunogen
Between 125 and 175
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse eIF4AIII/EIF4A3 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibody AAA212091 (lot AAA212091-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse eIF4AIII/EIF4A3 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibody AAA212091 (lot AAA212091-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human eIF4AIII/EIF4A3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibody AAA212091 (lot AAA212091-1) used for IP at 3 ug/mg lysate. eIF4AIII/EIF4A3 was also immunoprecipitated y rabbit anti-eIF4AIII/EIF4A3 antibody which recognizes a downstream epitope. For blotting immunoprecipitated eIF4AIII/EIF4A3, was used at 0.4 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human eIF4AIII/EIF4A3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibody AAA212091 (lot AAA212091-1) used for IP at 3 ug/mg lysate. eIF4AIII/EIF4A3 was also immunoprecipitated y rabbit anti-eIF4AIII/EIF4A3 antibody which recognizes a downstream epitope. For blotting immunoprecipitated eIF4AIII/EIF4A3, was used at 0.4 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
IHC (Immunohiostchemistry)
(Detection of human eIF4AIII/EIF4A3 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 (Cat. No. AAA212091 Lot1 left panel and Cat. No. Lot1 right panel) used at a dilution of 1:200 (1ug/ml) and 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human eIF4AIII/EIF4A3 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 (Cat. No. AAA212091 Lot1 left panel and Cat. No. Lot1 right panel) used at a dilution of 1:200 (1ug/ml) and 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse eIF4AIII/EIF4A3 by immunohistochemistry. Sample: FFPE section of mouse renal carcinoma. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 (Cat. No. AAA212091 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse eIF4AIII/EIF4A3 by immunohistochemistry. Sample: FFPE section of mouse renal carcinoma. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 (Cat. No. AAA212091 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-eIF4AIII/EIF4A3 antibody
Eukaryotic initiation factor 4A-III is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. eIF4AIII is an ATP-dependent helicase and a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs.
Product Categories/Family for anti-eIF4AIII/EIF4A3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,871 Da
NCBI Official Full Name
Homo sapiens eukaryotic translation initiation factor 4A3 (EIF4A3), mRNA
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4A3
NCBI Official Symbol
EIF4A3
NCBI Official Synonym Symbols
RCPS; DDX48; MUK34; NUK34; NMP265; eIF4AIII
NCBI Protein Information
eukaryotic initiation factor 4A-III; ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; NMP 265; eIF-4A-III; eIF4A-III; eukaryotic initiation factor 4A-like NUK-34; eukaryo
UniProt Protein Name
Eukaryotic initiation factor 4A-III
UniProt Gene Name
EIF4A3
UniProt Synonym Gene Names
DDX48; KIAA0111; eIF-4A-III; eIF4A-III; NMP 265; hNMP 265
UniProt Entry Name
IF4A3_HUMAN
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Product Notes
The eIF4AIII/EIF4A3 eif4a3 (Catalog #AAA212091) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-eIF4AIII/EIF4A3 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's eIF4AIII/EIF4A3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the eIF4AIII/EIF4A3 eif4a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF4AIII/EIF4A3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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