WB (Western Blot)
(Detection of human and mouse eIF4E by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody AAA211337 (lot AAA211337-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse eIF4E Polyclonal Antibody | anti-eIF4E antibody
Rabbit anti-eIF4E Antibody, Affinity Purified
Gene Names
EIF4E; CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
eIF4E, Antibody; Rabbit anti-eIF4E Antibody, Affinity Purified; CBP; EIF4F; eukaryotic translation initiation factor 4E-like 1; eukaryotic translation initiation factor 4E; mRNA cap-binding protein; eIF-4F 25 kDa subunit; EIF4EL1; EIF4E1; eIF4E; AUTS19; eIF-4E; Eukaryotic translation initiation factor 4E; anti-eIF4E antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-eIF4E antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, X. laevis, Bovine, Rabbit
Immunogen
Between 167 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse eIF4E by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody AAA211337 (lot AAA211337-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse eIF4E by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody AAA211337 (lot AAA211337-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human eIF4E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF4E antibody AAA211337 (lot AAA211337-3) used for IP at 3 ug per reaction. eIF4E was also immunoprecipitated by rabbit anti-eIF4E antibody For blotting immunoprecipitated eIF4E, AAA211337 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human eIF4E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF4E antibody AAA211337 (lot AAA211337-3) used for IP at 3 ug per reaction. eIF4E was also immunoprecipitated by rabbit anti-eIF4E antibody For blotting immunoprecipitated eIF4E, AAA211337 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Related Product Information for anti-eIF4E antibody
eIF4E is a component of the EIF4F initiation complex (eIF4A, eIF4E, eIF4G) that is involved in the processes of mRNA cap recognition, poly (A) binding, and the recruitment of mRNA to the ribosome during protein synthesis. eIF4E plays a role in binding the 5'cap of the mRNA for translation initiation.
Product Categories/Family for anti-eIF4E antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,260 Da
NCBI Official Full Name
eukaryotic translation initiation factor 4E isoform 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E
NCBI Official Symbol
EIF4E
NCBI Official Synonym Symbols
CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1
NCBI Protein Information
eukaryotic translation initiation factor 4E; eIF-4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; eukaryotic translation initiation factor 4E-like 1
UniProt Protein Name
Eukaryotic translation initiation factor 4E
UniProt Gene Name
EIF4E
UniProt Synonym Gene Names
EIF4EL1; EIF4F; eIF-4E; eIF4E
UniProt Entry Name
IF4E_HUMAN
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Product Notes
The eIF4E eif4e (Catalog #AAA211337) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-eIF4E Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's eIF4E can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the eIF4E eif4e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF4E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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