WB (Western Blot)
(Detection of human eiF4G1/eiF4GI by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody AAA210987 (lot AAA210987-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human eIF4G1/eIF4GI Polyclonal Antibody | anti-eIF4G1/eIF4GI antibody
Rabbit anti-eIF4G1/eIF4GI Antibody, Affinity Purified
Gene Names
EIF4G1; P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
eIF4G1/eIF4GI, Antibody; Rabbit anti-eIF4G1/eIF4GI Antibody, Affinity Purified; eIF-4-gamma 1; PARK18; P220; eukaryotic translation initiation factor 4 gamma 1; EIF4GI; EIF4-gamma; EIF-4G1; eIF-4G 1; EIF4G; EIF4F; eucaryotic translation initiation factor 4G; Eukaryotic translation initiation factor 4 gamma 1; anti-eIF4G1/eIF4GI antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-eIF4G1/eIF4GI antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 575 and 625
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human eiF4G1/eiF4GI by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody AAA210987 (lot AAA210987-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human eiF4G1/eiF4GI by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody AAA210987 (lot AAA210987-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human eiF4G1/eiF4GI by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody AAA210987 (lot AAA210987-2) used for IP at 6 ug per reaction. eiF4G1/eiF4GI was also immunoprecipitated by rabbit anti-eiF4G1/eiF4GI antibody For blotting immunoprecipitated eiF4G1/eiF4GI, rabbit anti-eiF4G1/eiF4GI antibody was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human eiF4G1/eiF4GI by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody AAA210987 (lot AAA210987-2) used for IP at 6 ug per reaction. eiF4G1/eiF4GI was also immunoprecipitated by rabbit anti-eiF4G1/eiF4GI antibody For blotting immunoprecipitated eiF4G1/eiF4GI, rabbit anti-eiF4G1/eiF4GI antibody was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-eIF4G1/eIF4GI antibody
Eukaryotic initiation factor 4 gamma 1 (eIF4G1) is one of 2 eIF4G proteins found in human (eIF4G1 and eIF4G3). eIF4G is a subunit of the eIF4F heterotrimeric complex composed of eIF4A, eIF4E, and eIF4G. eIF4F plays a role in recruiting the ribosome to the 5'-end of capped mRNAs during protein synthesis. In the eIF4F complex, eIF4G functions as a scaffold to bridge the cap-binding subunit, eIF4E, with the RNA helicase, eIF4A.
Product Categories/Family for anti-eIF4G1/eIF4GI antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
220
NCBI Official Full Name
eukaryotic translation initiation factor 4 gamma 1 isoform 4
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4 gamma 1
NCBI Official Symbol
EIF4G1
NCBI Official Synonym Symbols
P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1
NCBI Protein Information
eukaryotic translation initiation factor 4 gamma 1
UniProt Protein Name
Eukaryotic translation initiation factor 4 gamma 1
UniProt Gene Name
EIF4G1
UniProt Synonym Gene Names
EIF4F; EIF4G; EIF4GI; eIF-4-gamma 1; eIF-4G 1; eIF-4G1
UniProt Entry Name
IF4G1_HUMAN
Similar Products
Product Notes
The eIF4G1/eIF4GI eif4g1 (Catalog #AAA210987) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-eIF4G1/eIF4GI Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's eIF4G1/eIF4GI can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the eIF4G1/eIF4GI eif4g1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF4G1/eIF4GI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.