WB (Western Blot)
(Detection of human eIF4G3/eIF4GII by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, RKO, LNCaP, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4G3/eIF4GII antibody AAA211622 (lot AAA211622-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human eIF4G3/eIF4GII Polyclonal Antibody | anti-eIF4G3/eIF4GII antibody
Rabbit anti-eIF4G3/eIF4GII Antibody, Affinity Purified
Gene Names
EIF4G3; eIF4G 3; eIF4GII; eIF-4G 3
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
eIF4G3/eIF4GII, Antibody; Rabbit anti-eIF4G3/eIF4GII Antibody, Affinity Purified; eIF-4-gamma II; eukaryotic translation initiation factor 4 gamma 3; eIF4GII; eIF-4G 3; eIF4G 3; eIF-4-gamma 3; Eukaryotic translation initiation factor 4 gamma 3; anti-eIF4G3/eIF4GII antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-eIF4G3/eIF4GII antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 300 and 350
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human eIF4G3/eIF4GII by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, RKO, LNCaP, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4G3/eIF4GII antibody AAA211622 (lot AAA211622-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human eIF4G3/eIF4GII by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, RKO, LNCaP, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4G3/eIF4GII antibody AAA211622 (lot AAA211622-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human eIF4G3/eIF4GII by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF4G3/eIF4GII antibody AAA211622 (lot AAA211622-1) used for IP at 6 ug per reaction. eIF4G3/eIF4GII was also immunoprecipitated by anti-eIF4G3/eIF4GII antibodies and For blotting immunoprecipitated eIF4G3/eIF4GII, AAA211622 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human eIF4G3/eIF4GII by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF4G3/eIF4GII antibody AAA211622 (lot AAA211622-1) used for IP at 6 ug per reaction. eIF4G3/eIF4GII was also immunoprecipitated by anti-eIF4G3/eIF4GII antibodies and For blotting immunoprecipitated eIF4G3/eIF4GII, AAA211622 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-eIF4G3/eIF4GII antibody
Eukaryotic initiation factor 4 gamma 3 (eIF4G3) is one of 2 eIF4G proteins found in human (eIF4G1 and eIF4G3). eIF4G is a subunit of the eIF4F heterotrimeric complex composed of eIF4A, eIF4E, and eIF4G. eIF4F plays a role in recruiting the ribosome to the 5'-end of capped mRNAs during protein synthesis. In the eIF4F complex, eIF4G functions as a scaffold to bridge the cap-binding subunit, eIF4E, with the RNA helicase, eIF4A.
Product Categories/Family for anti-eIF4G3/eIF4GII antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
176,652 Da
NCBI Official Full Name
eukaryotic translation initiation factor 4 gamma 3 isoform 3
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4 gamma, 3
NCBI Official Symbol
EIF4G3
NCBI Official Synonym Symbols
eIF4G 3; eIF4GII; eIF-4G 3
NCBI Protein Information
eukaryotic translation initiation factor 4 gamma 3; eIF-4-gamma 3; eIF-4-gamma II; OTTHUMP00000002722
UniProt Protein Name
Eukaryotic translation initiation factor 4 gamma 3
UniProt Gene Name
EIF4G3
UniProt Synonym Gene Names
eIF-4-gamma 3; eIF-4G 3; eIF4G 3; eIF4GII
UniProt Entry Name
IF4G3_HUMAN
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Product Notes
The eIF4G3/eIF4GII eif4g3 (Catalog #AAA211622) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-eIF4G3/eIF4GII Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's eIF4G3/eIF4GII can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the eIF4G3/eIF4GII eif4g3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF4G3/eIF4GII, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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