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product-image-AAA212929_WB10.jpg WB (Western Blot) (Detection of human ELAC2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ELAC2 antibody AAA212929 (lot AAA212929-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

Rabbit anti-Human, Mouse ELAC2 Polyclonal Antibody | anti-ELAC2 antibody

Rabbit anti-ELAC2 Antibody, Affinity Purified

Gene Names
ELAC2; ELC2; HPC2; COXPD17
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ELAC2, Antibody; Rabbit anti-ELAC2 Antibody, Affinity Purified; putative prostate cancer susceptibility protein HPC2/ELAC2; zinc phosphodiesterase ELAC protein 2; tRNase Z 2; tRNase Z (long form); tRNA 3 endonuclease 2; RNase Z 2; ribonuclease Z 2; HPC2; heredity prostate cancer protein 2; ELC2; elaC-like protein 2; elaC homolog protein 2; COXPD17; elaC homolog 2; Zinc phosphodiesterase ELAC protein 2; elaC ribonuclease Z 2; anti-ELAC2 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ELAC2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Gorilla, Chimpanzee
Immunogen
Between 150 and 200
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human ELAC2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ELAC2 antibody AAA212929 (lot AAA212929-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

product-image-AAA212929_WB10.jpg WB (Western Blot) (Detection of human ELAC2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ELAC2 antibody AAA212929 (lot AAA212929-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IP (Immunoprecipitation)

(Detection of human ELAC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ELAC2 antibody AAA212929 (lot AAA212929-1) used for IP at 6 ug per reaction. ELAC2 was also immunoprecipitated by rabbit anti-ELAC2 antibody For blotting immunoprecipitated ELAC2, AAA212929 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

product-image-AAA212929_IP11.jpg IP (Immunoprecipitation) (Detection of human ELAC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ELAC2 antibody AAA212929 (lot AAA212929-1) used for IP at 6 ug per reaction. ELAC2 was also immunoprecipitated by rabbit anti-ELAC2 antibody For blotting immunoprecipitated ELAC2, AAA212929 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IHC (Immunohiostchemistry)

(Detection of mouse ELAC2 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-ELAC2 (Cat. No. AAA212929 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

product-image-AAA212929_IHC13.jpg IHC (Immunohiostchemistry) (Detection of mouse ELAC2 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-ELAC2 (Cat. No. AAA212929 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

IHC (Immunohistochemistry)

(Detection of human ELAC2 by immunohistochemistry. Sample: FFPE section of human stomach. Antibody: Affinity purified rabbit anti-ELAC2 (Cat. No. AAA212929 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

product-image-AAA212929_IHC15.jpg IHC (Immunohistochemistry) (Detection of human ELAC2 by immunohistochemistry. Sample: FFPE section of human stomach. Antibody: Affinity purified rabbit anti-ELAC2 (Cat. No. AAA212929 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-ELAC2 antibody
ELAC2 is a zinc phosphodiesterase, which displays mitochondrial tRNA 3'-processing endonuclease activity.It is involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BQ52].
Product Categories/Family for anti-ELAC2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
87,564 Da
NCBI Official Full Name
zinc phosphodiesterase ELAC protein 2 isoform 1
NCBI Official Synonym Full Names
elaC ribonuclease Z 2
NCBI Official Symbol
ELAC2
NCBI Official Synonym Symbols
ELC2; HPC2; COXPD17
NCBI Protein Information
zinc phosphodiesterase ELAC protein 2; RNase Z 2; tRNase Z 2; elaC homolog 2; ribonuclease Z 2; tRNase Z (long form); tRNA 3 endonuclease 2; elaC homolog protein 2; heredity prostate cancer protein 2; putative prostate cancer susceptibility protein HPC2/E
UniProt Protein Name
Zinc phosphodiesterase ELAC protein 2
UniProt Gene Name
ELAC2
UniProt Synonym Gene Names
HPC2; RNase Z 2
UniProt Entry Name
RNZ2_HUMAN

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Product Notes

The ELAC2 elac2 (Catalog #AAA212929) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ELAC2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ELAC2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ELAC2 elac2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ELAC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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