IHC (Immunohiostchemistry)
(Elafin/Skalp was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- Elafin/Skalp Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
Rabbit Elafin/Skalp Polyclonal Antibody | anti-PI3 antibody
Anti-Elafin/Skalp Antibody
Gene Names
PI3; ESI; WAP3; SKALP; WFDC14; cementoin
Reactivity
HumanNo cross reactivity with other proteins.
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Elafin/Skalp, Antibody; Anti-Elafin/Skalp Antibody; Cementoin; Elafin; ES 1; ESI; PI3; PI-3; PI 3; SKALP; Trappin 2; WAP3; WFDC14; P19957; peptidase inhibitor 3; anti-PI3 antibody
Host
Rabbit
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
117
Applicable Applications for anti-PI3 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human Elafin/Skalp recombinant protein (Position: A61-Q117).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Elafin/Skalp was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- Elafin/Skalp Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry)
(Elafin/Skalp was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- Elafin/Skalp Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
WB (Western Blot)
(Western blot analysis of Elafin/Skalp expression in human placenta extract (lane 1). Elafin/Skalp at 25KD was detected using rabbit anti- Elafin/Skalp Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.)
WB (Western Blot)
(Western blot analysis of Elafin/Skalp expression in human placenta extract (lane 1). Elafin/Skalp at 25KD was detected using rabbit anti- Elafin/Skalp Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.)
Related Product Information for anti-PI3 antibody
Rabbit IgG polyclonal antibody for Elafin (PI3) detection.
Background: Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoprotease (SKALP), is a protein that in humans is encoded by the PI3 gene. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. And this gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines.
Background: Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoprotease (SKALP), is a protein that in humans is encoded by the PI3 gene. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. And this gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines.
References
1. "Entrez Gene: PI3 peptidase inhibitor 3, skin-derived (SKALP)".
2. Clauss A, Lilja H, Lundwall A (Nov 2002). "A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein". Biochem J 368 (Pt 1): 233-42.
3. Molhuizen HO, Zeeuwen PL, Olde Weghuis D, Geurts van Kessel A, Schalkwijk J (Feb 1994). "Assignment of the human gene encoding the epidermal serine proteinase inhibitor SKALP (PI3) to chromosome region 20q12-->q13". Cytogenet Cell Genet 66 (2): 129-31.
2. Clauss A, Lilja H, Lundwall A (Nov 2002). "A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein". Biochem J 368 (Pt 1): 233-42.
3. Molhuizen HO, Zeeuwen PL, Olde Weghuis D, Geurts van Kessel A, Schalkwijk J (Feb 1994). "Assignment of the human gene encoding the epidermal serine proteinase inhibitor SKALP (PI3) to chromosome region 20q12-->q13". Cytogenet Cell Genet 66 (2): 129-31.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,270 Da
NCBI Official Full Name
elafin preproprotein
NCBI Official Synonym Full Names
peptidase inhibitor 3
NCBI Official Symbol
PI3
NCBI Official Synonym Symbols
ESI; WAP3; SKALP; WFDC14; cementoin
NCBI Protein Information
elafin
UniProt Protein Name
Elafin
UniProt Gene Name
PI3
UniProt Synonym Gene Names
WAP3; WFDC14; ESI; PI-3; SKALP
UniProt Entry Name
ELAF_HUMAN
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Product Notes
The PI3 pi3 (Catalog #AAA46624) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Elafin/Skalp Antibody reacts with Human No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Elafin/Skalp can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PI3 pi3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Elafin/Skalp, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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