Rabbit anti-Mouse, Rat Elastase/ELANE/ELA2 Polyclonal Antibody | anti-ELANE antibody

Anti-Elastase/ELANE/ELA2 Antibody

Cat. Num. AAA46832

• Gene Names: Elane; NE; Ela2; F430011M15Rik
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: Elastase/ELANE/ELA2, Antibody; Anti-Elastase/ELANE/ELA2 Antibody; ELA2; ELANE; Elastase 2; Elastase-2; HLE; HNE; Leukocyte elastase; Medullasin; Neutrophil elastase; PMN E; PMN elastase; SCN1; P08246; elastase, neutrophil expressed; anti-ELANE antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 265

• Applicable Applications for anti-ELANE antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Notes: Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.; Other applications have not been tested.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: E. coli-derived mouse Elastase/ELANE/ELA2 recombinant protein (Position: S27-N265). Mouse Elastase/ELANE/ELA2 shares 92% amino acid (aa) sequence identity with rat Elastase/ELANE/ELA2.
• Preparation and Storage: Store at -20°C. After reconstitution, at 4°C. It can also be aliquotted and stored frozen at -20°C for a longer time.; Avoid repeated freezing and thawing.

IHC (Immunohistochemisry)

(Elastase/ELANE/ELA2 was detected in paraffin-embedded sections of rat spleen tissues using rabbit anti- Elastase/ELANE/ELA2 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohiostchemistry)

(Elastase/ELANE/ELA2 was detected in paraffin-embedded sections of mouse spleen tissues using rabbit anti- Elastase/ELANE/ELA2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

WB (Western Blot)

(Western blot analysis of Elastase/ELANE/ELA2 expression in mouse bone extract (lane 1) and mouse spleen extract (lane 2). Elastase/ELANE/ELA2 at 29KD was detected using rabbit anti- Elastase/ELANE/ELA2 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)

Related Product Information for anti-ELANE antibody

Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

References

1. Ancliff, P. J., Gale, R. E., Liesner, R., Hann, I. M., Linch, D. C.Mutations in the ELA2 gene encoding neutrophil elastase are present in most patients with sporadic severe congenital neutropenia but only in some patients with the familial form of the disease. Blood 98: 2645-2650, 2001.
2. Bristow, C. L., Fiscus, S. A., Flood, P. M., Arnold, R. R. Inhibition of HIV-1 by modification of a host membrane protease. Int. Immun. 7: 239-249, 1995.

NCBI and Uniprot Product Information

• NCBI GI #: 114052444
• NCBI GeneID: 50701
• NCBI Accession #: NP_056594.2
• NCBI GenBank Nucleotide #: NM_015779.2
• UniProt Accession #: Q3UP87; Q61515; A6H698
• Molecular Weight: 28,648 Da
• NCBI Official Full Name: neutrophil elastase
• NCBI Official Synonym Full Names: elastase, neutrophil expressed
• NCBI Official Symbol: Elane
• NCBI Official Synonym Symbols: NE; Ela2; F430011M15Rik
• NCBI Protein Information: neutrophil elastase
• UniProt Protein Name: Neutrophil elastase
• UniProt Gene Name: Elane
• UniProt Synonym Gene Names: Ela2