WB (Western Blot)
(Sample: Lung (Mouse) Lysate at 30 ug. Primary: Anti- alpha Elastin/Tropoelastin at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution. Predicted band size: 70 kD. Observed band size: 68 kD.)
Rabbit Elastin Polyclonal Antibody | anti-Elastin antibody
Elastin
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Protein A affinity purified.
Synonyms
Elastin, Antibody; Elastin; alpha Elastin/Tropoelastin; Elastin isoform a; ELN; ELN_HUMAN; elastin isoform m precursor; FLJ38671; FLJ43523; Supravalvular aortic stenosis; Tropoelastin; Williams Beuren syndrome; Williams syndrome region; ADCL1; SVAS; WBS; WS; anti-Elastin antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A affinity purified.
Form/Format
0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-Elastin antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Conjugation
FITC
Immunogen
KLH conjugated synthetic peptide derived from human Elastin 681-786/786
Positive control
Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
Subcellular location
Secreted, extracellular space, extracellular matrix. Note=Extracellular matrix of elastic fibers.
Preparation and Storage
Store at -20 degree C for one year. Avoid repeated freeze/thaw cycles
WB (Western Blot)
(Sample: Lung (Mouse) Lysate at 30 ug. Primary: Anti- alpha Elastin/Tropoelastin at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution. Predicted band size: 70 kD. Observed band size: 68 kD.)
WB (Western Blot)
(Sample: Lung (Mouse) Lysate at 30 ug. Primary: Anti- alpha Elastin/Tropoelastin at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution. Predicted band size: 70 kD. Observed band size: 68 kD.)
IHC (Immunohistochemistry)
(Images provided the Independent Validation Program:Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti-alpha Elastin Polyclonal Antibody at 1:1000 4 degree C temperature overnight 4 degree C followed by conjugation to secondary antibody.)
IHC (Immunohistochemistry)
(Images provided the Independent Validation Program:Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti-alpha Elastin Polyclonal Antibody at 1:1000 4 degree C temperature overnight 4 degree C followed by conjugation to secondary antibody.)
Related Product Information for anti-Elastin antibody
Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
70 kDa
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Product Notes
The Elastin (Catalog #AAA268990) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Elastin reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Elastin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Elastin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Elastin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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