FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NCI-H292 cells using ELF4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ELF4 Polyclonal Antibody | anti-ELF4 antibody
ELF4 Antibody (Center)
Gene Names
ELF4; MEF; ELFR
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ELF4, Antibody; ELF4 Antibody (Center); ETS-related transcription factor Elf-4; E74-like factor 4; Myeloid Elf-1-like factor; ELF4 {ECO:0000312|EMBL:CAI428821}; anti-ELF4 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ELF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-237 amino acids from the Central region of human ELF4.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
211-237
Sequence Length
663
Applicable Applications for anti-ELF4 antibody
WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NCI-H292 cells using ELF4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NCI-H292 cells using ELF4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(ELF4 Antibody (Center) western blot analysis in HL-60,K562 cell line lysates (35ug/lane).This demonstrates the ELF4 antibody detected the ELF4 protein (arrow).)
WB (Western Blot)
(ELF4 Antibody (Center) western blot analysis in HL-60,K562 cell line lysates (35ug/lane).This demonstrates the ELF4 antibody detected the ELF4 protein (arrow).)
Related Product Information for anti-ELF4 antibody
ELF4 is transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. The protein acts synergistically with RUNX1 to transactivate the IL3 promoter. Also transactivates the PRF1 promoter in natural killer (NK) cells. This protein plays a role in the development and function of NK and NK T-cells and in innate immunity.
Product Categories/Family for anti-ELF4 antibody
References
Miyazaki Y., Sun X.Oncogene 13:1721-1729(1996)
Aryee D.N.T., Petermann R.Gene 210:71-78(1998)
Mao S., Frank R.C., Zhang J.Mol. Cell. Biol. 19:3635-3644(1999)
Aryee D.N.T., Petermann R.Gene 210:71-78(1998)
Mao S., Frank R.C., Zhang J.Mol. Cell. Biol. 19:3635-3644(1999)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70730
NCBI Official Full Name
ETS-related transcription factor Elf-4
NCBI Official Synonym Full Names
E74-like factor 4 (ets domain transcription factor)
NCBI Official Symbol
ELF4
NCBI Official Synonym Symbols
MEF; ELFR
NCBI Protein Information
ETS-related transcription factor Elf-4
UniProt Protein Name
ETS-related transcription factor Elf-4
UniProt Gene Name
ELF4
UniProt Entry Name
ELF4_HUMAN
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Product Notes
The ELF4 elf4 (Catalog #AAA288091) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 211-237. The ELF4 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's ELF4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the ELF4 elf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ELF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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