IF (Immunofluorescence)
(Immunofluorescent analysis of ELF5 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Rabbit ELF5 Polyclonal Antibody | anti-ELF5 antibody
Anti-ELF5 Antibody
Gene Names
ELF5; ESE2
Reactivity
Human, Mouse, Rat, Bovine
Applications
Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
ELF5, Antibody; Anti-ELF5 Antibody; ESE2; ETS-related transcription factor Elf-5; E74-like factor 5; Epithelium-restricted ESE-1-related Ets factor; Epithelium-specific Ets transcription factor 2; ESE-2; anti-ELF5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of ELF5 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
160
Applicable Applications for anti-ELF5 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ELF5. The exact sequence is proprietary.
Preparation and Storage
Upon receipt aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of ELF5 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
IF (Immunofluorescence)
(Immunofluorescent analysis of ELF5 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
WB (Western Blot)
(Western blot analysis of ELF5 expression in HeLa (A), HuvEc (B), Jurkat (C) whole cell lysates.)
WB (Western Blot)
(Western blot analysis of ELF5 expression in HeLa (A), HuvEc (B), Jurkat (C) whole cell lysates.)
Related Product Information for anti-ELF5 antibody
Rabbit polyclonal antibody to ELF5
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ETS-related transcription factor Elf-5 isoform 3
NCBI Official Synonym Full Names
E74-like factor 5 (ets domain transcription factor)
NCBI Official Symbol
ELF5
NCBI Official Synonym Symbols
ESE2
NCBI Protein Information
ETS-related transcription factor Elf-5; epithelium-restricted ESE-1-related Ets factor; epithelium-specific Ets transcription factor 2
UniProt Protein Name
ETS-related transcription factor Elf-5
UniProt Gene Name
ELF5
UniProt Synonym Gene Names
ESE2; ESE-2
UniProt Entry Name
ELF5_HUMAN
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Product Notes
The ELF5 elf5 (Catalog #AAA104343) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ELF5 Antibody reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's ELF5 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the ELF5 elf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ELF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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