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IP (Immunoprecipitation)
(Immunoprecipitating EMD in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of AAA232471 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA232471 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
Rabbit anti-Human, Mouse EMD Polyclonal Antibody | anti-EMD antibody
EMD Antibody
Gene Names
EMD; STA; EDMD; LEMD5
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
EMD, Antibody; EMD Antibody; Emerin; EMD; EDMD; STA; anti-EMD antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
254
Applicable Applications for anti-EMD antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant Human Emerin protein (1-222AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-15378 / sc-8084 / sc-8085 / sc-8086
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IP (Immunoprecipitation)
(Immunoprecipitating EMD in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of AAA232471 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA232471 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
IP (Immunoprecipitation)
(Immunoprecipitating EMD in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of AAA232471 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA232471 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA232471 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA232471 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human bladder cancer using AAA232471 at dilution of 1:100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human bladder cancer using AAA232471 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human heart tissue using AAA232471 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human heart tissue using AAA232471 at dilution of 1:100)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate,A549 whole cell lysate,Colo320 whole cell lysate,HepG2 whole cell lysate,Jurkat whole cell lysate,MCF-7 whole cell lysate,Mouse lung tissueAll lanes: EMD antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 29 KDaObserved band size: 35 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate,A549 whole cell lysate,Colo320 whole cell lysate,HepG2 whole cell lysate,Jurkat whole cell lysate,MCF-7 whole cell lysate,Mouse lung tissueAll lanes: EMD antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 29 KDaObserved band size: 35 KDa)
Related Product Information for anti-EMD antibody
Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,994 Da
NCBI Official Full Name
emerin
NCBI Official Synonym Full Names
emerin
NCBI Official Symbol
EMD
NCBI Official Synonym Symbols
STA; EDMD; LEMD5
NCBI Protein Information
emerin
UniProt Protein Name
Emerin
UniProt Gene Name
EMD
UniProt Synonym Gene Names
EDMD; STA
UniProt Entry Name
EMD_HUMAN
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Product Notes
The EMD emd (Catalog #AAA232471) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EMD Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EMD can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the EMD emd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EMD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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