WB (Western Blot)
(Western blot analysis of Mouse kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-403). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-403) at 1:1000.)
Rabbit anti-Rat, Mouse ENaC Alpha Polyclonal Antibody | anti-Scnn1a antibody
ENaC Alpha Antibody: RPE
Reactivity
Rat, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
ENaC Alpha, Antibody; ENaC Alpha Antibody: RPE; Scnn1a; anti-Scnn1a antibody
Host
Rabbit
Reactivity
Rat, Mouse
Clonality
Polyclonal
Form/Format
Affinity Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
699
Applicable Applications for anti-Scnn1a antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Conjugate
RPE
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide.
Preparation and Storage
-20 degree C
WB (Western Blot)
(Western blot analysis of Mouse kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-403). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-403) at 1:1000.)
WB (Western Blot)
(Western blot analysis of Mouse kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-403). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-403) at 1:1000.)
WB (Western Blot)
(Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-403). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-403) at 1:1000.)
WB (Western Blot)
(Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-403). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-403) at 1:1000.)
Related Product Information for anti-Scnn1a antibody
Background Info: Detects ~85kDa
Scientific Background: The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
Scientific Background: The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
Product Categories/Family for anti-Scnn1a antibody
References
1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689. 2. Gu Y. (2008) J Cell Physiol. 216(2):453-457. 3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609. 4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594. 5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45. 6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79,019 Da
NCBI Official Full Name
amiloride-sensitive sodium channel subunit alpha
NCBI Official Synonym Full Names
sodium channel, non-voltage-gated 1 alpha subunit
NCBI Official Symbol
Scnn1a
NCBI Protein Information
amiloride-sensitive sodium channel subunit alpha; SCNEA; alpha-ENaC; alpha-NaCH; epithelial Na(+) channel subunit alpha; epithelial sodium channel alpha subunit; sodium channel, nonvoltage-gated 1 alpha; nonvoltage-gated sodium channel 1 subunit alpha; sodium channel, nonvoltage-gated, type I, alpha
UniProt Protein Name
Amiloride sensitive sodium channel alpha1 subunit
UniProt Gene Name
Scnn1a
UniProt Synonym Gene Names
rCG_30182
UniProt Entry Name
Q6IRJ1_RAT
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Product Notes
The Scnn1a scnn1a (Catalog #AAA103612) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENaC Alpha Antibody: RPE reacts with Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ENaC Alpha can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Scnn1a scnn1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENaC Alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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