WB (Western Blot)
(Detection of human and mouse ENH/PDLIM5 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-ENH/PDLIM5 antibody AAA211596 (lot AAA211596-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human, Mouse ENH/PDLIM5 Polyclonal Antibody | anti-ENH/PDLIM5 antibody
Rabbit anti-ENH/PDLIM5 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ENH/PDLIM5, Antibody; Rabbit anti-ENH/PDLIM5 Antibody, Affinity Purified; ENH; PDZ and LIM domain protein 5; LIM; L9; enigma-like PDZ and LIM domains protein; enigma-like LIM domain protein; ENH1; enigma homolog; PDZ and LIM domain 5; anti-ENH/PDLIM5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-ENH/PDLIM5 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 546 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse ENH/PDLIM5 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-ENH/PDLIM5 antibody AAA211596 (lot AAA211596-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human and mouse ENH/PDLIM5 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-ENH/PDLIM5 antibody AAA211596 (lot AAA211596-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human ENH/PDLIM5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-ENH/PDLIM5 antibody AAA211596 (lot AAA211596-2) used for IP at 6 ug per reaction. ENH/PDLIM5 was also immunoprecipitated by rabbit anti-ENH/PDLIM5 antibody For blotting immunoprecipitated ENH/PDLIM5, AAA211596 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human ENH/PDLIM5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-ENH/PDLIM5 antibody AAA211596 (lot AAA211596-2) used for IP at 6 ug per reaction. ENH/PDLIM5 was also immunoprecipitated by rabbit anti-ENH/PDLIM5 antibody For blotting immunoprecipitated ENH/PDLIM5, AAA211596 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-ENH/PDLIM5 antibody
The PDZ and LIM domain 5 protein (PDLIM5) bears a PDZ (post-synaptic density-95/discs large/zone occludens-1) domain and three LIM (Lin-11 Isl-1 mec03) zinc-binding domains. PDLIM5 may function as an adaptor protein that mediates protein-protein interactions and interactions with the actin cytoskeleton. PDLIM5 has been implicated in the development of schizophrenia and bipolar disorder.
Product Categories/Family for anti-ENH/PDLIM5 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The ENH/PDLIM5 pdlim5 (Catalog #AAA211596) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ENH/PDLIM5 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ENH/PDLIM5 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ENH/PDLIM5 pdlim5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENH/PDLIM5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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