Rabbit ENO3 Polyclonal Antibody | anti-ENO3 antibody

Anti-ENO3 Antibody, Rabbit Polyclonal

Cat. Num. AAA255048

• Gene Names: Eno3; MSE; Eno-3
• Reactivity: Mouse; Predicted to React with: Human, Rat, Bovin, Pig (Species predicted to react based on 100% sequence homology)
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: ENO3, Antibody; Anti-ENO3 Antibody, Rabbit Polyclonal; ENO3/beta-enolase Antibody, Rabbit PAb, Antigen Affinity Purified; enolase 3 (beta, muscle); Anti-Eno-3 Antibody; Anti-MSE Antibody; anti-ENO3 antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted to React with: Human, Rat, Bovin, Pig (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse ENO3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ENO3 antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse ENO3/beta-enolase
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse ENO3/beta-enolase, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(ENO3 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg NIH-3T3 Whole Cell LysateLane C:0.5 mg RAW246.7 Whole Cell Lysate4 uL anti-ENO3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ENO3 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 50 kDaObserved band size :54 kDa)

WB (Western Blot)

(Anti-ENO3 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Molt-4 Whole Cell LysateLane C: NIH-3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:47 kDaObserved band size:47 kDa)

Related Product Information for anti-ENO3 antibody

ENO3 is one of the three enolase isoenzymes found in mammals. As a homodimer, ENO3 is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for ENO3 gene that differ only in their 5' UTR. ENO3 may play a role in muscle development and regeneration. It appears to have a function in striated muscle development and regeneration.

References

Peshavaria M, et al. (1989) Structure of human muscle (beta) enolase mRNA and protein deduced from a genomic clone. Nucleic Acids Res. 17(21):8862. Cali L, et al. (1990) Nucleotide sequence of a cDNA encoding the human muscle-specific enolase (MSE). Nucleic Acids Res. 18(7):1893. Peshavaria M, et al. (1991) Molecular structure of the human muscle-specific enolase gene (ENO3). Biochem J. 275(2):427-33.

NCBI and Uniprot Product Information

• NCBI GI #: 209862931
• NCBI GeneID: 13808
• NCBI Accession #: NP_001129534.1
• NCBI GenBank Nucleotide #: NM_001136062.2
• UniProt Accession #: P21550
• Molecular Weight: 47,025 Da
• NCBI Official Full Name: beta-enolase
• NCBI Official Synonym Full Names: enolase 3, beta muscle
• NCBI Official Symbol: Eno3
• NCBI Official Synonym Symbols: MSE; Eno-3
• NCBI Protein Information: beta-enolase; muscle-specific enolase; skeletal muscle enolase; 2-phospho-D-glycerate hydro-lyase
• UniProt Protein Name: Beta-enolase
• UniProt Gene Name: Eno3
• UniProt Synonym Gene Names: Eno-3; MSE
• UniProt Entry Name: ENOB_MOUSE