FCM (Flow Cytometry)
(ENOA Antibody (N-term) (Catalog Number: AAA28805) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ENOA Polyclonal Antibody | anti-ENOA antibody
ENOA Antibody (N-term)
Gene Names
ENO1; NNE; PPH; MPB1; ENO1L1; HEL-S-17
Reactivity
Human, Mouse, RatPredicted: Bovine, Monkey
Applications
Immunofluorescence, Western Blot, Flow Cytometry, ELISA
Purity
Purified Rabbit Polyclnal Antibody (Pab)
Synonyms
ENOA, Antibody; ENOA Antibody (N-term); ENO1; ENO1L1; MBPB1; MPB1; Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; Phosphopyruvate hydratase; Plasminogen-binding protein; anti-ENOA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Monkey
Predicted: Bovine, Monkey
Clonality
Polyclonal
Isotype
IgG
Specificity
This ENOA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-60 amino acids from the N-terminal region of human ENOA.
Purity/Purification
Purified Rabbit Polyclnal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
33-60
Applicable Applications for anti-ENOA antibody
Provided below are standard protocols that you may find useful for product applications.
Immunofluorescence, Western Blot(WB), Flow Cytometry, ELISA (EIA).
Immunofluorescence, Western Blot(WB), Flow Cytometry, ELISA (EIA).
Application Notes
IF~1:10-50
WB~~1:1000
FC~1:10~50
WB~~1:1000
FC~1:10~50
Function
Glycolytic enzyme the catalyzes the conversion of 2- phosphoglycerate to phosphoenolpyruvate (PubMed:29775581, PubMed:1369209). In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:2005901, PubMed:10802057, PubMed:12666133, PubMed:29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133). Stimulates immunoglobulin production (PubMed:1369209).
Cellular Location
Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line
Tissue Location
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM (Flow Cytometry)
(ENOA Antibody (N-term) (Catalog Number: AAA28805) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM (Flow Cytometry)
(ENOA Antibody (N-term) (Catalog Number: AAA28805) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of lysates from mouse brain tissue, mouse NIH/3T3, U-251 MG,mouse C2C12 cell line (from left to right), using ENOA Antibody (N-term) (Catalog Number: AAA28805). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from mouse brain tissue, mouse NIH/3T3, U-251 MG,mouse C2C12 cell line (from left to right), using ENOA Antibody (N-term) (Catalog Number: AAA28805). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with ENOA Antibody (N-term) (Catalog Number: AAA28805). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENOA primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ENOA immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with ENOA Antibody (N-term) (Catalog Number: AAA28805). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENOA primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ENOA immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
Related Product Information for anti-ENOA antibody
Background: ENO1 is one of three enolase isoenzymes found in mammals; the protein alpha-enolase, a homodimeric soluble enzyme, and is also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter
References
Cappello,P., Int. J. Cancer 125 (3), 639-648 (2009). Wygrecka,M., Blood 113 (22), 5588-5598 (2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW Human = 47, 37; Mouse = 47; Rat = 47 KDa
NCBI Official Full Name
c-myc promoter-binding protein-1 isoform MBP-1
NCBI Official Synonym Full Names
enolase 1
NCBI Official Symbol
ENO1
NCBI Official Synonym Symbols
NNE; PPH; MPB1; ENO1L1; HEL-S-17
NCBI Protein Information
alpha-enolase; c-myc promoter-binding protein-1
UniProt Protein Name
Alpha-enolase
UniProt Gene Name
ENO1
UniProt Synonym Gene Names
ENO1L1; MBPB1; MPB1; NNE
UniProt Entry Name
ENOA_HUMAN
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Product Notes
The ENOA eno1 (Catalog #AAA28805) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 33-60. The ENOA Antibody (N-term) reacts with Human, Mouse, Rat Predicted: Bovine, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's ENOA can be used in a range of immunoassay formats including, but not limited to, Provided below are standard protocols that you may find useful for product applications. Immunofluorescence, Western Blot(WB), Flow Cytometry, ELISA (EIA). IF~1:10-50 WB~~1:1000 FC~1:10~50. Researchers should empirically determine the suitability of the ENOA eno1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENOA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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