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product-image-AAA286136_FCM11.jpg FCM/FACS (Flow Cytometry) (ENTPD3 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Rabbit anti-Human ENTPD3 Polyclonal Antibody | anti-ENTPD3 antibody

ENTPD3 Antibody (C-term)

Gene Names
ENTPD3; HB6; CD39L3; NTPDase-3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ENTPD3, Antibody; ENTPD3 Antibody (C-term); Ectonucleoside triphosphate diphosphohydrolase 3; NTPDase 3; CD39 antigen-like 3; Ecto-ATP diphosphohydrolase 3; Ecto-ATPDase 3; Ecto-ATPase 3; Ecto-apyrase 3; HB6; ENTPD3; CD39L3; anti-ENTPD3 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ENTPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 496-527 amino acids from the C-terminal region of human ENTPD3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
496-527
Sequence Length
452
Applicable Applications for anti-ENTPD3 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(ENTPD3 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA286136_FCM11.jpg FCM/FACS (Flow Cytometry) (ENTPD3 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(ENTPD3 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENTPD3 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

product-image-AAA286136_IHC13.jpg IHC (Immunohiostchemistry) (ENTPD3 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENTPD3 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(ENTPD3 Antibody (C-term) western blot analysis in K562 and HL-60 cell line lysates (35ug/lane).This demonstrates the ENTPD3 antibody detected the ENTPD3 protein (arrow).)

product-image-AAA286136_WB15.jpg WB (Western Blot) (ENTPD3 Antibody (C-term) western blot analysis in K562 and HL-60 cell line lysates (35ug/lane).This demonstrates the ENTPD3 antibody detected the ENTPD3 protein (arrow).)
Related Product Information for anti-ENTPD3 antibody
ENTPD3 is similar to E-type nucleotidases (NTPases).
NTPases, such as CD39, mediate catabolism of extracellular
nucleotides. ENTPD3 contains 4 apyrase-conserved regions which is
characteristic of NTPases.
Product Categories/Family for anti-ENTPD3 antibody
References
Fausther, M., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 298 (6), L804-L818 (2010) :
Gaddie, K.J., et al. Biochemistry 48(40):9437-9447(2009)
Munkonda, M.N., et al. FEBS J. 276(2):479-496(2009)
Munkonda, M.N., et al. Biochem. Pharmacol. 74(10):1524-1534(2007)
Ivanenkov, V.V., et al. Biochemistry 44(25):8998-9012(2005)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
956
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59105
NCBI Official Full Name
ectonucleoside triphosphate diphosphohydrolase 3 isoform 1
NCBI Official Synonym Full Names
ectonucleoside triphosphate diphosphohydrolase 3
NCBI Official Symbol
ENTPD3
NCBI Official Synonym Symbols
HB6; CD39L3; NTPDase-3
NCBI Protein Information
ectonucleoside triphosphate diphosphohydrolase 3
UniProt Protein Name
Ectonucleoside triphosphate diphosphohydrolase 3
UniProt Gene Name
ENTPD3
UniProt Synonym Gene Names
CD39L3; NTPDase 3; Ecto-ATPDase 3; Ecto-ATPase 3
UniProt Entry Name
ENTP3_HUMAN

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Product Notes

The ENTPD3 entpd3 (Catalog #AAA286136) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 496-527. The ENTPD3 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENTPD3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ENTPD3 entpd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENTPD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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