Chicken EOMES Polyclonal Antibody | anti-EOMES antibody

EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

Cat. Num. AAA26890

• Gene Names: Eomes; Tbr2; TBR-2; C77258
• Reactivity: Mouse, Human, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: EOMES, Antibody; EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2); anti-EOMES antibody

• Host: Chicken
• Reactivity: Mouse, Human, Rat
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes mouse Tbr2 observed at ~85/95kD. Target bands appear larger than the calculated molecular size of 74.8kD (isoform 1) and 72.7kD (isoform 2). Species Crossreactivity: human and rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-EOMES antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 1:1000 detected Tbr2 in mouse and human brain. Requires HIER with citrate buffer pH 6.0; WB: 0.05ug/ml detected Tbr2 in 10ug of E13-14 mouse brain lysates.; Applications are based on unconjugated antibody.
• Immunogen: Linear peptide corresponding to mouse EOMES, conjugated to KLH. Epitope: C-Terminus.
• Conjugate: MaxLight550
• Positive Control: E13-14 mouse brain lysate
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

IHC (Immunohistchemistry)

(Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)

IHC (Immunohistochemistry)

(Paraffin-embedded mouse cerebellum was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using 1:100 dilution. Immuno-reactivity is mostly nuclear.)

IHC (Immunohistochemistry)

(Paraffin-embedded mouse olfactory lobe was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using a 1:100 dilution. Reactivity is mostly nuclear.)

IS (Immunostaining)

(Paraffin-embedded human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Staining pattern appears as cytoplasmic.)

IS (Immunostaining)

(Paraffin-embedded mouse brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Staining pattern appears as cytoplasmic.)

WB (Western Blot)

(Western Blot Analysis: Representative lot data. E13-14 mouse brain lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with (1:10,000) dilution. Proteins were visualized using a Rabbit Anti-Chicken conjugated to HRP and a chemi-luminescence detection system. Arrow indicates Tbr2 (~73 kD).)

Product Categories/Family for anti-EOMES antibody

Antibodies; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 83921572
• NCBI GeneID: 13813
• NCBI Accession #: NP_034266
• NCBI GenBank Nucleotide #: NM_010136
• UniProt Accession #: O54839
• Molecular Weight: 75kDa
• NCBI Official Full Name: eomesodermin homolog isoform 1
• NCBI Official Synonym Full Names: eomesodermin
• NCBI Official Symbol: Eomes
• NCBI Official Synonym Symbols: Tbr2; TBR-2; C77258
• NCBI Protein Information: eomesodermin homolog
• UniProt Protein Name: Eomesodermin homolog
• UniProt Gene Name: Eomes
• UniProt Synonym Gene Names: Tbr2; T-brain-2; TBR-2
• UniProt Entry Name: EOMES_MOUSE