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product-image-AAA232473_IF10.jpg IF (Immunofluorescence) (Immunofluorescent analysis of A549 cells using AAA232473 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Rabbit anti-Human, Mouse EPHA1 Polyclonal Antibody | anti-EPHA1 antibody

EPHA1 Antibody

Gene Names
EPHA1; EPH; EPHT; EPHT1
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
EPHA1, Antibody; EPHA1 Antibody; Ephrin type-A receptor 1; EPH tyrosine kinase; EPH tyrosine kinase 1; Erythropoietin-producing hepatoma receptor; Tyrosine-protein kinase recept; EPHA1; EPH; EPHT; EPHT1; anti-EPHA1 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
976
Applicable Applications for anti-EPHA1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human Ephrin type-A receptor 1 protein (296-414AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-925 / sc-20738
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

IF (Immunofluorescence)

(Immunofluorescent analysis of A549 cells using AAA232473 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

product-image-AAA232473_IF10.jpg IF (Immunofluorescence) (Immunofluorescent analysis of A549 cells using AAA232473 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemisry)

(Immunohistochemistry of paraffin-embedded human liver cancer using AAA232473 at dilution of 1:100)

product-image-AAA232473_IHC11.jpg IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human liver cancer using AAA232473 at dilution of 1:100)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human gastric cancer using AAA232473 at dilution of 1:100)

product-image-AAA232473_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using AAA232473 at dilution of 1:100)

WB (Western Blot)

(Western BlotPositive WB detected in:Mouse brain tissueAll lanes: EPHA1 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 109,53,52 kDaObserved band size: 109,30 kDa)

product-image-AAA232473_WB15.jpg WB (Western Blot) (Western BlotPositive WB detected in:Mouse brain tissueAll lanes: EPHA1 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 109,53,52 kDaObserved band size: 109,30 kDa)
Related Product Information for anti-EPHA1 antibody
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,069 Da
NCBI Official Full Name
ephrin type-A receptor 1
NCBI Official Synonym Full Names
EPH receptor A1
NCBI Official Symbol
EPHA1
NCBI Official Synonym Symbols
EPH; EPHT; EPHT1
NCBI Protein Information
ephrin type-A receptor 1
UniProt Protein Name
Ephrin type-A receptor 1
UniProt Gene Name
EPHA1
UniProt Synonym Gene Names
EPH; EPHT; EPHT1; hEpha1
UniProt Entry Name
EPHA1_HUMAN

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Product Notes

The EPHA1 epha1 (Catalog #AAA232473) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EPHA1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EPHA1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the EPHA1 epha1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPHA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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