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IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with EPHA10 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit EPHA10 Polyclonal Antibody | anti-EPHA10 antibody
EPHA10 Antibody (N-term)
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
EPHA10, Antibody; EPHA10 Antibody (N-term); Ephrin type-A receptor 10; EPHA10; anti-EPHA10 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EPHA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-179 amino acids from the N-terminal region of human EPHA10.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
149-179
Sequence Length
892
Applicable Applications for anti-EPHA10 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with EPHA10 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with EPHA10 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of EPHA10 (arrow) using rabbit polyclonal EPHA10 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA10 gene (Lane 2) (Origene Technologies).(2ug/ml))
WB (Western Blot)
(Western blot analysis of EPHA10 (arrow) using rabbit polyclonal EPHA10 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA10 gene (Lane 2) (Origene Technologies).(2ug/ml))
WB (Western Blot)
(Western blot analysis of EPHA10 Antibody (N-term) in K562 cell line lysates (35ug/lane). EPHA10 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of EPHA10 Antibody (N-term) in K562 cell line lysates (35ug/lane). EPHA10 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of lysates from Hela, 293T cell line (from left to right), using EPHA10 Antibody (N-term). AAA284431 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Hela, 293T cell line (from left to right), using EPHA10 Antibody (N-term). AAA284431 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Related Product Information for anti-EPHA10 antibody
EPHA10 is a receptor for members of the ephrin-A family. It binds to EFNA3, EFNA4 and EFNA5.
Product Categories/Family for anti-EPHA10 antibody
References
Aasheim,H.C., Biochim. Biophys. Acta 1723 (1-3), 1-7 (2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
109716
NCBI Official Full Name
ephrin type-A receptor 10 isoform 3
NCBI Official Synonym Full Names
EPH receptor A10
NCBI Official Symbol
EPHA10
NCBI Protein Information
ephrin type-A receptor 10
UniProt Protein Name
Ephrin type-A receptor 10
UniProt Gene Name
EPHA10
UniProt Entry Name
EPHAA_HUMAN
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Product Notes
The EPHA10 epha10 (Catalog #AAA284431) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 149-179. The EPHA10 Antibody (N-term) reacts with Human (Predicted Reactivity: Chicken, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's EPHA10 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the EPHA10 epha10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPHA10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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