IF (Immunofluorescence)
(Immunofluorescent analysis of EPHA3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).)
Rabbit EPHA3 Polyclonal Antibody | anti-EPHA3 antibody
Anti-EPHA3 Antibody
Gene Names
EPHA3; EK4; ETK; HEK; ETK1; HEK4; TYRO4
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Pig, Zebrafish
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
EPHA3, Antibody; Anti-EPHA3 Antibody; ETK; ETK1; HEK; TYRO4; Ephrin type-A receptor 3; EPH-like kinase 4; EK4; hEK4; Human embryo kinase; Tyrosine-protein kinase TYRO4; Tyrosine-protein kinase receptor ETK1; Eph-like tyrosine kinase 1; anti-EPHA3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Pig, Zebrafish
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of EPHA3 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
983
Applicable Applications for anti-EPHA3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: (1/500 - 1/1000)
IHC: (1/50 - 1/100)
IF/ICC: (1/50 - 1/200)
IHC: (1/50 - 1/100)
IF/ICC: (1/50 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EPHA3. The exact sequence is proprietary.
Preparation and Storage
Upon receipt aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of EPHA3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of EPHA3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of EPHA3 staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of EPHA3 staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Western blot analysis of EPHA3 expression in mouse liver (A), mouse kidney (B) whole cell lysates.)
WB (Western Blot)
(Western blot analysis of EPHA3 expression in mouse liver (A), mouse kidney (B) whole cell lysates.)
Related Product Information for anti-EPHA3 antibody
Rabbit polyclonal antibody to EPHA3
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Band Size: 110kD ; Observed Band SIze: 110kD
NCBI Official Full Name
ephrin type-A receptor 3 isoform a
NCBI Official Synonym Full Names
EPH receptor A3
NCBI Official Symbol
EPHA3
NCBI Official Synonym Symbols
EK4; ETK; HEK; ETK1; HEK4; TYRO4
NCBI Protein Information
ephrin type-A receptor 3; EPH-like kinase 4; human embryo kinase 1; eph-like tyrosine kinase 1; TYRO4 protein tyrosine kinase; tyrosine-protein kinase receptor ETK1
UniProt Protein Name
Ephrin type-A receptor 3
UniProt Gene Name
EPHA3
UniProt Synonym Gene Names
ETK; ETK1; HEK; TYRO4; EK4; hEK4; Human embryo kinase; Eph-like tyrosine kinase 1
UniProt Entry Name
EPHA3_HUMAN
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Product Notes
The EPHA3 epha3 (Catalog #AAA17837) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EPHA3 Antibody reacts with Human, Mouse, Rat, Bovine, Chicken, Dog, Pig, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's EPHA3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: (1/500 - 1/1000) IHC: (1/50 - 1/100) IF/ICC: (1/50 - 1/200). Researchers should empirically determine the suitability of the EPHA3 epha3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPHA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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