Rabbit EphA7 Polyclonal Antibody | anti-EPHA7 antibody

EphA7 Antibody (N-term)

Cat. Num. AAA288791

• Gene Names: EPHA7; EHK3; EK11; EHK-3; HEK11
• Reactivity: Human; Predicted Reactivity: Mouse, Rat
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: EphA7, Antibody; EphA7 Antibody (N-term); Ephrin type-A receptor 7; EPH homology kinase 3; EHK-3; EPH-like kinase 11; EK11; hEK11; EPHA7; EHK3; HEK11; anti-EPHA7 antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This EphA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-41 amino acids from the N-terminal region of human EphA7.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 11-41
• Sequence Length: 998

• Applicable Applications for anti-EPHA7 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
• Function: Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7.
• Cellular Location: Cell membrane; Single-pass type I membrane protein
• Tissue Location: Widely expressed.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with EphA7 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of A375 cells using EphA7 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-EPHA7 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Product Categories/Family for anti-EPHA7 antibody

Cardiovascular; Neuroscience

References

Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001).
Xu, Q., et al., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 355(1399):993-1002 (2000).
Holder, N., et al., Development 126(10):2033-2044 (1999).
Zhou, R., Pharmacol. Ther. 77(3):151-181 (1998).
Fox, G.M., et al., Oncogene 10(5):897-905 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 4758282
• NCBI GeneID: 2045
• NCBI Accession #: Q15375
• NCBI GenBank Nucleotide #: NM_004440.3
• NCBI Official Full Name: ephrin type-A receptor 7 isoform 1
• NCBI Official Synonym Full Names: EPH receptor A7
• NCBI Official Symbol: EPHA7
• NCBI Official Synonym Symbols: EHK3; EK11; EHK-3; HEK11
• NCBI Protein Information: ephrin type-A receptor 7
• UniProt Protein Name: Ephrin type-A receptor 7
• UniProt Gene Name: EPHA7
• UniProt Synonym Gene Names: EHK3; HEK11; EHK-3; EK11; hEK11
• UniProt Entry Name: EPHA7_HUMAN