Rabbit EPHB1/2 Polyclonal Antibody | anti-EPHB1/2 antibody

Phospho-EPHB1/2 (Tyr778/Tyr780) Antibody

Cat. Num. AAA329906

• Gene Names: EPHB1; ELK; NET; Hek6; EPHT2
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (88%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: EPHB1/2, Antibody; Phospho-EPHB1/2 (Tyr778/Tyr780) Antibody; Cek 6; EK6; ELK; Elkh; EpHreceptor B1; EpHtyrosine kinase 2; EPH-like kinase 6; Ephb1; EPHB1_HUMAN; Ephrin type B receptor 1; Ephrin type-B receptor 1; EPHT2; HEK 6; HEK6; NET; Neuronally-expressed EPH-related tyrosine kinase; soluble EPHB1 variant 1; Tyrosine protein kinase receptor EpH2; Tyrosine-protein kinase receptor EPH-2; cek5; Developmentally regulated EpHrelated tyrosine kinase; DRT; EK5; ELK related protein tyrosine kinase; EpHreceptor B2; EpHtyrosine kinase 3; EPH-like kinase 5; EPHB2; EPHB2_HUMAN; Ephrin type B receptor 2; Ephrin type-B receptor 2; EPHT 3; ERK; ETECK; hEK5; Nuk; Prkm 5; Receptor protein tyrosine kinase HEK 5; Renal carcinoma antigen NY-REN-47; Sek 3; Tyro 5; Tyrosine protein kinase receptor CEK 5; Tyrosine protein kinase receptor EpH3; Tyrosine protein kinase receptor QEK 5; Tyrosine-protein kinase receptor EPH-3; Tyrosine-protein kinase TYRO5; anti-EPHB1/2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (88%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-EPHB1/2 (Tyr778/Tyr780) Antibody detects endogenous levels of EPHB1/2 only when phosphorylated at Tyr778/Tyr780.; Tissue Specificity: Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-EPHB1/2 antibody: ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human EPHB1/2 around the phosphorylation site of Tyr778/Tyr780.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Autophosphorylated; ligand binding stimulates autophosphorylation on tyrosine residues.Polyubiquitinated; ligand binding stimulates ubiquitination.Ligand binding induces cleavage by matrix metalloproteinases (MMPs) such as MMP7/MMP9, producing an EphB2/N-terminal fragment (NTF) and a C-terminal long fragment (EphB2-LF). EphB2-LF is further cleaved by MMPs, producing EphB2/CTF1 which is further cleaved by the PS1/gamma-secretase producing EphB2/CTF2.
• Subunit Structure: Heterotetramer upon binding of the ligand (By similarity). The heterotetramer is composed of an ephrin dimer and a receptor dimer. Interacts (via PDZ-binding motif) with GRIP1 and PICK1 (via PDZ domain) (By similarity). Interacts with ARHGEF15; mediates ARHGEF15 phosphorylation, ubiquitination and degradation by the proteasome (By similarity). Interacts with AQP1; involved in endolympHproduction in the inner ear (By similarity). Interacts with SPSB1 and SPSB4. The phosphorylated form interacts with RASA1 (via SH2 domain 1) (By similarity). Interacts with EFNA5 (By similarity).
• Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
• Subcellular Location: Cell membrane>Single-pass type I membrane protein. Cell projection>Axon. Cell projection>Dendrite.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IF (Immunofluorescence)

(Staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of EPHB1/2 (Phospho-Tyr778/Tyr780) using serum treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-EPHB1/2 antibody

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolympHproduction. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. May be involved in the regulation of platelet activation and blood coagulation.

NCBI and Uniprot Product Information

• NCBI GI #: 4758284
• NCBI GeneID: 2047
• NCBI Accession #: NP_004432.1
• NCBI GenBank Nucleotide #: NM_004441.4
• UniProt Accession #: P54762; P29323
• Molecular Weight: 109,885 Da
• NCBI Official Full Name: ephrin type-B receptor 1
• NCBI Official Synonym Full Names: EPH receptor B1
• NCBI Official Symbol: EPHB1
• NCBI Official Synonym Symbols: ELK; NET; Hek6; EPHT2
• NCBI Protein Information: ephrin type-B receptor 1; EK6; EPH-like kinase 6; eph tyrosine kinase 2; soluble EPHB1 variant 1; tyrosine-protein kinase receptor EPH-2; neuronally-expressed EPH-related tyrosine kinase
• UniProt Protein Name: Ephrin type-B receptor 1
• UniProt Gene Name: EPHB1
• UniProt Synonym Gene Names: ELK; EPHT2; HEK6; NET; EK6; hEK6; NET
• UniProt Entry Name: EPHB1_HUMAN