IF (Immunofluorescence)
(Confocal immunofluorescent analysis of EPOR Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human EPOR Polyclonal Antibody | anti-EPOR antibody
EPOR Antibody (Center)
Gene Names
EPOR; EPO-R
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
EPOR, Antibody; EPOR Antibody (Center); Erythropoietin receptor; EPO-R; EPOR; anti-EPOR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EPOR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-357 amino acids from the Central region of human EPOR.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.3 (varies by lot)
Sequence Positions
329-357
Sequence Length
508
Applicable Applications for anti-EPOR antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of EPOR Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of EPOR Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
FCM/FACS (Flow Cytometry)
(EPOR Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(EPOR Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(EPOR Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the EPOR antibody detected the EPOR protein (arrow).)
WB (Western Blot)
(EPOR Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the EPOR antibody detected the EPOR protein (arrow).)
Related Product Information for anti-EPOR antibody
This gene encodes the erythropoietin receptor which is a
member of the cytokine receptor family. Upon erythropoietin
binding, this receptor activates Jak2 tyrosine kinase which
activates different intracellular pathways including: Ras/MAP
kinase, phosphatidylinositol 3-kinase and STAT transcription
factors. The stimulated erythropoietin receptor appears to have a
role in erythroid cell survival. Defects in the erythropoietin
receptor may produce erythroleukemia and familial erythrocytosis.
Dysregulation of this gene may affect the growth of certain tumors.
Alternate splicing results in multiple transcript variants.
member of the cytokine receptor family. Upon erythropoietin
binding, this receptor activates Jak2 tyrosine kinase which
activates different intracellular pathways including: Ras/MAP
kinase, phosphatidylinositol 3-kinase and STAT transcription
factors. The stimulated erythropoietin receptor appears to have a
role in erythroid cell survival. Defects in the erythropoietin
receptor may produce erythroleukemia and familial erythrocytosis.
Dysregulation of this gene may affect the growth of certain tumors.
Alternate splicing results in multiple transcript variants.
Product Categories/Family for anti-EPOR antibody
References
Lim, A.C., et al. Biochemistry 49(18):3797-3804(2010)
Perrotta, S., et al. PLoS ONE 5 (8), E12015 (2010) :
Khankin, E.V., et al. PLoS ONE 5 (2), E9246 (2010) :
Wincewicz, A., et al. Folia Histochem. Cytobiol. 47(3):425-430(2009)
Ketteler, R., et al. J. Biol. Chem. 278(4):2654-2660(2003)
Perrotta, S., et al. PLoS ONE 5 (8), E12015 (2010) :
Khankin, E.V., et al. PLoS ONE 5 (2), E9246 (2010) :
Wincewicz, A., et al. Folia Histochem. Cytobiol. 47(3):425-430(2009)
Ketteler, R., et al. J. Biol. Chem. 278(4):2654-2660(2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55065
NCBI Official Full Name
erythropoietin receptor
NCBI Official Synonym Full Names
erythropoietin receptor
NCBI Official Symbol
EPOR
NCBI Official Synonym Symbols
EPO-R
NCBI Protein Information
erythropoietin receptor
UniProt Protein Name
Erythropoietin receptor
UniProt Gene Name
EPOR
UniProt Synonym Gene Names
EPO-R
UniProt Entry Name
EPOR_HUMAN
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Product Notes
The EPOR epor (Catalog #AAA283620) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 329-357. The EPOR Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EPOR can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the EPOR epor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPOR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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