Rabbit EPRS1/PARS Polyclonal Antibody | anti-EPRS1 antibody

Anti-EPRS1/PARS Antibody

Cat. Num. AAA19268

• Gene Names: EPRS; EARS; PARS; QARS; QPRS; PIG32; GLUPRORS
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: EPRS1/PARS, Antibody; Anti-EPRS1/PARS Antibody; EPRS; GLNS; PARS; QARS; QPRS; PIG32; Bifunctional glutamate/proline--tRNA ligase; Bifunctional aminoacyl-tRNA synthetase; Cell proliferation-inducing gene 32 protein; Glutamatyl-prolyl-tRNA synthetase [Includes: Glutamate--tRNA ligase; EC 6. 1. 1. 17; Glutamyl-tRNA synthetase; GluRS; Proline--tRNA ligase; EC 6. 1. 1. 15; Prolyl-tRNA synthetase]; glutamyl-prolyl-tRNA synthetase 1; anti-EPRS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rabbit IgG polyclonal antibody for EPRS1/PARS detection.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

• Applicable Applications for anti-EPRS1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml|Human, Mouse, Rat|; IHC-P: 2-5ug/ml|Human, Mouse, Rat|; ICC/IF: 5ug/ml|Human|; FC/FACS/FCM: 1-3ug/1x106 cells|Human|; Direct ELISA: 0.1-0.5ug/ml|Human|
• Immunogen: E Coli-derived human EPRS1/PARS recombinant protein (Position: R1298-Y1512).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Recommended Detection Systems
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

FCM (Flow Cytometry)

(Figure 11. Flow Cytometry analysis of THP-1 cells using anti-EPRS1/PARS antibody (AAA19268).Overlay histogram showing THP-1 cells stained with AAA19268 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPRS1/PARS Antibody (AAA19268, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence)

(Figure 10. IF analysis of EPRS1/PARS using anti- EPRS1/PARS antibody (AAA19268).EPRS1/PARS was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- EPRS1/PARS Antibody (AAA19268) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry)

(Figure 9. IHC analysis of EPRS1/PARS using anti-EPRS1/PARS antibody (AAA19268).EPRS1/PARS was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EPRS1/PARS Antibody (AAA19268) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 8. IHC analysis of EPRS1/PARS using anti-EPRS1/PARS antibody (AAA19268).EPRS1/PARS was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EPRS1/PARS Antibody (AAA19268) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 7. IHC analysis of EPRS1/PARS using anti-EPRS1/PARS antibody (AAA19268).EPRS1/PARS was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EPRS1/PARS Antibody (AAA19268) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry)

(Figure 6. IHC analysis of EPRS1/PARS using anti-EPRS1/PARS antibody (AAA19268).EPRS1/PARS was detected in paraffin-embedded section of human gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EPRS1/PARS Antibody (AAA19268) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 5. IHC analysis of EPRS1/PARS using anti-EPRS1/PARS antibody (AAA19268).EPRS1/PARS was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EPRS1/PARS Antibody (AAA19268) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 4. IHC analysis of EPRS1/PARS using anti-EPRS1/PARS antibody (AAA19268).EPRS1/PARS was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EPRS1/PARS Antibody (AAA19268) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 3. IHC analysis of EPRS1/PARS using anti-EPRS1/PARS antibody (AAA19268).EPRS1/PARS was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EPRS1/PARS Antibody (AAA19268) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 2. IHC analysis of EPRS1/PARS using anti-EPRS1/PARS antibody (AAA19268).EPRS1/PARS was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EPRS1/PARS Antibody (AAA19268) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot)

(Figure 1. Western blot analysis of EPRS1/PARS using anti-EPRS1/PARS antibody (AAA19268).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human Hek293 whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: rat pancreas tissue lysatesLane 5: mouse pancreas tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-EPRS1/PARS antigen affinity purified polyclonal antibody (Catalog # AAA19268) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for EPRS1/PARS at approximately 170-180KD. The expected band size for EPRS1/PARS is at 171KD.)

Related Product Information for anti-EPRS1 antibody

Bifunctional aminoacyl-tRNA synthetase is an enzyme that in humans is encoded by the EPRS gene. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined.

References

1. Arif, A., Jia, J., Mukhopadhyay, R., Willard, B., Kinter, M., Fox, P. L. Two-site phosphorylation of EPRS coordinates multinodal regulation of noncanonical translational control activity. Molec. Cell 35: 164-180, 2009.
2. Arif, A., Terenzi, F., Potdar, A. A., Jia, J., Sacks, J., China, A., Halawani, D., Vasu, K., Li, X., Brown, J. M., Chen, J., Kozma, S. C., Thomas, G., Fox, P. L. EPRS is a critical mTORC1-S6K1 effector that influences adiposity in mice. Nature 542: 357-361, 2017.
3. Cerini, C., Kerjan, P., Astier, M., Gratecos, D., Mirande, M., Semeriva, M. A component of the multisynthetase complex is a multifunctional aminoacyl-tRNA synthetase. EMBO J. 10: 4267-4277, 1991.

NCBI and Uniprot Product Information

• NCBI GI #: 62241042
• NCBI GeneID: 2058
• NCBI Accession #: NP_004437.2
• NCBI GenBank Nucleotide #: NM_004446.2
• UniProt Accession #: P07814; Q05BP6; Q05DF8; Q5DSM1; Q5H9S5; Q6PD57; Q86X73; A0AVA9; B9EGH3
• Molecular Weight: 170,591 Da
• NCBI Official Full Name: bifunctional glutamate/proline--tRNA ligase
• NCBI Official Synonym Full Names: glutamyl-prolyl-tRNA synthetase
• NCBI Official Symbol: EPRS
• NCBI Official Synonym Symbols: EARS; PARS; QARS; QPRS; PIG32; GLUPRORS
• NCBI Protein Information: bifunctional glutamate/proline--tRNA ligase
• UniProt Protein Name: Bifunctional glutamate/proline--tRNA ligase
• UniProt Gene Name: EPRS
• UniProt Synonym Gene Names: GLNS; PARS; QARS; QPRS; GluRS