WB (Western Blot)
(Detection of human EPS15 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EPS15 antibody AAA211609 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. EPS15 was also immunoprecipitated by rabbit anti-EPS15 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).)
Rabbit anti-Human EPS15 Polyclonal Antibody | anti-EPS15 antibody
Rabbit anti-EPS15 Antibody, Affinity Purified
Gene Names
EPS15; AF1P; AF-1P; MLLT5
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
EPS15, Antibody; Rabbit anti-EPS15 Antibody, Affinity Purified; AF-1P; ALL1 fused gene from chromosome 1; epidermal growth factor receptor substrate 15; MLLT5; protein AF-1p; Protein Eps15; AF1P; Epidermal growth factor receptor substrate 15; epidermal growth factor receptor pathway substrate 15; anti-EPS15 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-EPS15 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 575 and 625
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human EPS15 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EPS15 antibody AAA211609 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. EPS15 was also immunoprecipitated by rabbit anti-EPS15 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).)
WB (Western Blot)
(Detection of human EPS15 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EPS15 antibody AAA211609 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. EPS15 was also immunoprecipitated by rabbit anti-EPS15 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).)
IHC (Immunohistochemistry)
(Detection of human EPS15 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EPS15 (Cat. No. AAA211609) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human EPS15 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EPS15 (Cat. No. AAA211609) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-EPS15 antibody
EPS15 (epidermal growth factor receptor substrate 15) is a protein phosphorylated by the EGF receptor and involved in the internalization of the EGF-receptor via clathrin-coated pits.
Product Categories/Family for anti-EPS15 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
98,656 Da
NCBI Official Full Name
epidermal growth factor receptor substrate 15 isoform A
NCBI Official Synonym Full Names
epidermal growth factor receptor pathway substrate 15
NCBI Official Symbol
EPS15
NCBI Official Synonym Symbols
AF1P; AF-1P; MLLT5
NCBI Protein Information
epidermal growth factor receptor substrate 15; protein AF-1p; ALL1 fused gene from chromosome 1
UniProt Protein Name
Epidermal growth factor receptor substrate 15
UniProt Gene Name
EPS15
UniProt Synonym Gene Names
AF1P; Protein Eps15
UniProt Entry Name
EPS15_HUMAN
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Product Notes
The EPS15 eps15 (Catalog #AAA211609) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-EPS15 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EPS15 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the EPS15 eps15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPS15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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