<
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using EPHX2 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
Rabbit EPXH2 Polyclonal Antibody | anti-EPHX2 antibody
EPXH2 Polyclonal Antibody
Gene Names
EPHX2; CEH; SEH
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity Purification
Synonyms
EPXH2, Antibody; EPXH2 Polyclonal Antibody; CEH; SHE; Epoxide Hydratase; anti-EPHX2 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3.
Sequence Length
555
Applicable Applications for anti-EPHX2 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-240 of human EPHX2 (NP_001970.2)
Cross/Species Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using EPHX2 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using EPHX2 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using EPHX2 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3- conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using EPHX2 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3- conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cells using EPHX2 Rabbit pAb (A1885) at dilution of 1:200 (40x lens). Secondary antibody: Cy3- conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cells using EPHX2 Rabbit pAb (A1885) at dilution of 1:200 (40x lens). Secondary antibody: Cy3- conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of various lysates using EPHX2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of various lysates using EPHX2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using EPHX2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using EPHX2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)
Related Product Information for anti-EPHX2 antibody
This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes,binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described.
Product Categories/Family for anti-EPHX2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
63 kDa
NCBI Official Full Name
Bifunctional epoxide hydrolase 2
NCBI Official Synonym Full Names
epoxide hydrolase 2, cytoplasmic
NCBI Official Symbol
EPHX2
NCBI Official Synonym Symbols
CEH; SEH
NCBI Protein Information
bifunctional epoxide hydrolase 2; epoxide hydratase; epoxide hydrolase, soluble; epoxide hydrolase 2, cytosolic
UniProt Protein Name
Bifunctional epoxide hydrolase 2
UniProt Gene Name
EPHX2
UniProt Synonym Gene Names
CEH; SEH
UniProt Entry Name
HYES_HUMAN
Similar Products
Product Notes
The EPHX2 ephx2 (Catalog #AAA37262) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's EPXH2 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the EPHX2 ephx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPXH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.