IHC (Immunohistochemistry)
(Immunohistochemistry: Lung tissue (FFPE) stained with Rabbit anti-ER81 (Cat# AAA14073) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Rabbit ER81 Polyclonal Antibody | anti-ER81 antibody
Rabbit anti ER81 Polyclonal Antibody
Gene Names
ETV1; ER81
Reactivity
Mouse, Human, Rat
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Western Blot, Immunoprecipitation
Purity
Rabbit IgG is purified by Epitope Affinity Purification
Synonyms
ER81, Antibody; Rabbit anti ER81 Polyclonal Antibody; Ets-related transcription factor ER81; Ets variant protein ER81, Ets variant gene1; anti-ER81 antibody
Host
Rabbit
Reactivity
Mouse, Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody recognizes ~56 kDa of ER81 protein. This antibody also reacts with human, mouse, and rat. The other species are not tested.
Purity/Purification
Rabbit IgG is purified by Epitope Affinity Purification
Form/Format
The affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Applicable Applications for anti-ER81 antibody
ELISA (EIA), Flow Cytometry (FC), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/mL
Immunohistochemistry: 2-10 ug/ml
Western Blot: 0.1-1 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
Other species are not tested.
Immunoprecipitation: 2-5 ug/mL
Immunohistochemistry: 2-10 ug/ml
Western Blot: 0.1-1 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
Other species are not tested.
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Preparation
A synthetic peptide corresponding to the C-terminus of human ER81 protein. This sequence is identical within human, rat, mouse, bovine, dog and chicken.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at –20°C to –70°C. The antibodies can be stored at 2°C-8°C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.
IHC (Immunohistochemistry)
(Immunohistochemistry: Lung tissue (FFPE) stained with Rabbit anti-ER81 (Cat# AAA14073) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
IHC (Immunohistochemistry)
(Immunohistochemistry: Lung tissue (FFPE) stained with Rabbit anti-ER81 (Cat# AAA14073) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
WB (Western Blot)
(WB: The tissue lysate derived from mouse brain was immunoprobed by Rabbit anti- ER81 (AAA14073) at 1:500. An around ~56kDa (lane 1). This band is blocked by pre-incubation with immunizing peptide (Lane 2))
WB (Western Blot)
(WB: The tissue lysate derived from mouse brain was immunoprobed by Rabbit anti- ER81 (AAA14073) at 1:500. An around ~56kDa (lane 1). This band is blocked by pre-incubation with immunizing peptide (Lane 2))
Related Product Information for anti-ER81 antibody
The ETS transcription factors Ets2, ER81 and Elk1 belong to the Ets transcription factor family. ER81 (also designated ETV1) binds the motif 5'-CGGAA/T-3'. It is a prerequisite for normal development. An abnormal regulation contributes to neoplasia. ER81 is highly expressed in brain, testis, lung and heart. ER81 is also moderately expressed in spleen, pancreas, colon and small intestine. During development, ER81, PEA3 and ERM display unique expression patterns which suggest these transcriptional factors play an important role in organogenesis. ERK-1 activates ER81 transcriptional activity while MAPKAP kinase 2 inhibits ER81. ER81 also can be acetylated at N-terminus (Lys33 and Lys116).
References
Martine Pastorcic, et al. Ets transcription factors ER81 and Elk1 regulate the transcription of the human presenilin 1 gene promoter. Molecular Brain Research, 113 (1-2), 57-66, 2003.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62 kDa
NCBI Official Full Name
ETS translocation variant 1 isoform d
NCBI Official Synonym Full Names
ETS variant 1
NCBI Official Symbol
ETV1
NCBI Official Synonym Symbols
ER81
NCBI Protein Information
ETS translocation variant 1
UniProt Protein Name
ETS translocation variant 1
UniProt Gene Name
ETV1
UniProt Synonym Gene Names
ER81
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Product Notes
The ER81 etv1 (Catalog #AAA14073) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti ER81 Polyclonal Antibody reacts with Mouse, Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ER81 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP). ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/mL Immunohistochemistry: 2-10 ug/ml Western Blot: 0.1-1 ug/ml Optimal dilutions should be determined by researchers for the specific applications. Other species are not tested. Researchers should empirically determine the suitability of the ER81 etv1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ER81, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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