WB (Western Blot)
(Detection of human and mouse ERCC3 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ERCC3 antibody AAA211431 (lot AAA211431-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse ERCC3 Polyclonal Antibody | anti-ERCC3 antibody
Rabbit anti-ERCC3 Antibody, Affinity Purified
Gene Names
ERCC3; XPB; BTF2; GTF2H; RAD25; TFIIH
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ERCC3, Antibody; Rabbit anti-ERCC3 Antibody, Affinity Purified; TFIIH; basic transcription factor 2 89 kDa subunit; xeroderma pigmentosum, complementation group B; XPB; xeroderma pigmentosum group B-complementing protein; TTD2; TFIIH subunit XPB; TFIIH p89; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH 89 kDa subunit; DNA excision repair protein ERCC-3; BTF2 p89; RAD25; BTF2; DNA repair helicase; DNA repair protein complementing XP-B cells; excision repair cross-complementation group 3; excision repair cross-complementing rodent repair deficiency, complementation group 3; general transcription and DNA repair factor IIH helicase subunit XPB; GTF2H; ERCC excision repair 3, TFIIH core complex helicase subunit; anti-ERCC3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ERCC3 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine, Orangutan
Immunogen
Between 732 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse ERCC3 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ERCC3 antibody AAA211431 (lot AAA211431-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse ERCC3 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ERCC3 antibody AAA211431 (lot AAA211431-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human ERCC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERCC3 antibody AAA211431 (lot AAA211431-2) used for IP at 3 ug per reaction. ERCC3 was also immunoprecipitated by rabbit anti-ERCC3 antibody For blotting immunoprecipitated ERCC3, AAA211431 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human ERCC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERCC3 antibody AAA211431 (lot AAA211431-2) used for IP at 3 ug per reaction. ERCC3 was also immunoprecipitated by rabbit anti-ERCC3 antibody For blotting immunoprecipitated ERCC3, AAA211431 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-ERCC3 antibody
ERCC3 is a component of the core TFIIH basal transcription factor and functions as an ATP-dependent 3'-5' DNA helicase. ERCC3 is involved in nucleotide excision repair and is the cause of xeroderma pigmentosum complementation group B (XPB), also known as xeroderma pigmentosum II (XP2). Additionally, it has been found to be the cuase of Cockayne syndrome, and trichothiodystrophy (TTD). XPB is an autosomal recessive disease characterized by skin photosensitivity and a predisposition to skin cancer, and neurological abnormalities. Cockayne syndrome and TTD are similarly characterized by photosensitivity and neural abnormalities.
Product Categories/Family for anti-ERCC3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89,278 Da
NCBI Official Full Name
Homo sapiens excision repair cross-complementation group 3 (ERCC3), transcript variant 1, mRNA
NCBI Official Synonym Full Names
excision repair cross-complementation group 3
NCBI Official Symbol
ERCC3
NCBI Official Synonym Symbols
XPB; BTF2; GTF2H; RAD25; TFIIH
NCBI Protein Information
TFIIH basal transcription factor complex helicase XPB subunit; BTF2 p89; DNA excision repair protein ERCC-3; DNA repair helicase; DNA repair protein complementing XP-B cells; TFIIH 89 kDa subunit; TFIIH basal transcription factor complex 89 kDa subunit; T
UniProt Protein Name
TFIIH basal transcription factor complex helicase XPB subunit
UniProt Gene Name
ERCC3
UniProt Synonym Gene Names
XPB; XPBC; BTF2 p89; TFIIH 89 kDa subunit; TFIIH p89
UniProt Entry Name
ERCC3_HUMAN
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Product Notes
The ERCC3 ercc3 (Catalog #AAA211431) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ERCC3 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ERCC3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ERCC3 ercc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERCC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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