WB (Western Blot)
(Western blot analysis on NIH-3T3 cell lysate using ERD23 Antibody)
Rabbit anti-Human, Mouse ERD23 Polyclonal Antibody | anti-ERD23 antibody
ERD23 Antibody
Gene Names
KDELR3; ERD2L3
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ERD23, Antibody; ERD23 Antibody; ER lumen protein retaining receptor 3; ERD2L3; KDEL (Lys Asp Glu Leu) endoplasmic reticulum protein retention receptor 3; KDEL endoplasmic reticulum protein retention receptor 3; KDEL receptor 3; KDELR 3; anti-ERD23 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ERD23 antibody detects endogenous levels of ERD23
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
214
Applicable Applications for anti-ERD23 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human ERD23
Subcellular Location
Endoplasmic Reticulum Membrane; Multipass Membrane Protein.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (91%), Xenopus (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis on NIH-3T3 cell lysate using ERD23 Antibody)
WB (Western Blot)
(Western blot analysis on NIH-3T3 cell lysate using ERD23 Antibody)
IF (Immunofluorescence)
(AAA321224 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA321224 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-ERD23 antibody
Description: KDELR3 Required for the retention of luminal endoplasmic reticulum proteins. Determines the specificity of the luminal ER protein retention system. Also required for normal vesicular traffic through the Golgi. This receptor recognizes K-D-E-L. Belongs to the ERD2 family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Function: Required for the retention of luminal endoplasmic reticulum proteins. Determines the specificity of the luminal ER protein retention system. Also required for normal vesicular traffic through the Golgi. This receptor recognizes K-D-E-L (By similarity).
Similarity: Belongs to the ERD2 family.
Function: Required for the retention of luminal endoplasmic reticulum proteins. Determines the specificity of the luminal ER protein retention system. Also required for normal vesicular traffic through the Golgi. This receptor recognizes K-D-E-L (By similarity).
Similarity: Belongs to the ERD2 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 28 kDa
Predicted: 26 kDa
Predicted: 26 kDa
NCBI Official Full Name
ER lumen protein-retaining receptor 3 isoform a
NCBI Official Synonym Full Names
KDEL endoplasmic reticulum protein retention receptor 3
NCBI Official Symbol
KDELR3
NCBI Official Synonym Symbols
ERD2L3
NCBI Protein Information
ER lumen protein-retaining receptor 3
UniProt Protein Name
ER lumen protein-retaining receptor 3
UniProt Gene Name
KDELR3
UniProt Synonym Gene Names
KDEL receptor 3
Similar Products
Product Notes
The ERD23 kdelr3 (Catalog #AAA321224) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERD23 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ERD23 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the ERD23 kdelr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERD23, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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