FCM/FACS (Flow Cytometry)
(ERO1LB Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse ERO1LB Polyclonal Antibody | anti-ERO1LB antibody
ERO1LB Antibody (C-term)
Gene Names
ERO1B; ERO1LB; Ero1beta
Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ERO1LB, Antibody; ERO1LB Antibody (C-term); ERO1-like protein beta; ERO1-L-beta; 184-; Endoplasmic reticulum oxidoreductin-1-like protein B; Oxidoreductin-1-L-beta; ERO1LB; anti-ERO1LB antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ERO1LB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-293 amino acids from the C-terminal region of human ERO1LB.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.33 (varies by lot)
Sequence Positions
266-293
Sequence Length
467
Applicable Applications for anti-ERO1LB antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ERO1LB Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ERO1LB Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(ERO1LB Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ERO1LB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ERO1LB Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ERO1LB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(ERO1LB Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the ERO1LB antibody detected the ERO1LB protein (arrow).)
WB (Western Blot)
(ERO1LB Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the ERO1LB antibody detected the ERO1LB protein (arrow).)
Related Product Information for anti-ERO1LB antibody
ERO1LB is essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. ERO1LB acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. ERO1LB does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent.
Product Categories/Family for anti-ERO1LB antibody
References
Landa, I., et al. PLoS Genet. 5 (9), E1000637 (2009) Savu, M.K., et al. Surg Obes Relat Dis 5(2):172-180(2009) Tavendale, R., et al. J. Allergy Clin. Immunol. 121(4):860-863(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53543
NCBI Official Full Name
ERO1-like protein beta
NCBI Official Synonym Full Names
endoplasmic reticulum oxidoreductase beta
NCBI Official Symbol
ERO1B
NCBI Official Synonym Symbols
ERO1LB; Ero1beta
NCBI Protein Information
ERO1-like protein beta
UniProt Protein Name
ERO1-like protein beta
UniProt Gene Name
ERO1LB
UniProt Synonym Gene Names
ERO1-L-beta
UniProt Entry Name
ERO1B_HUMAN
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Product Notes
The ERO1LB ero1lb (Catalog #AAA287955) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 266-293. The ERO1LB Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ERO1LB can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ERO1LB ero1lb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERO1LB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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