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IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ESRRA Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Rabbit ESRRA Polyclonal Antibody | anti-ESRRA antibody
ESRRA Antibody (Center)
Gene Names
ESRRA; ERR1; ERRa; ESRL1; NR3B1; ERRalpha
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ESRRA, Antibody; ESRRA Antibody (Center); Steroid hormone receptor ERR1; Estrogen receptor-like 1; Estrogen-related receptor alpha; ERR-alpha; Nuclear receptor subfamily 3 group B member 1; ESRRA; ERR1; ESRL1; NR3B1; anti-ESRRA antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ESRRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-159 amino acids from the Central region of human ESRRA.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
131-159
Sequence Length
422
Applicable Applications for anti-ESRRA antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ESRRA Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ESRRA Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(Western blot analysis of ESRRA (arrow) using rabbit polyclonal ESRRA Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ESRRA gene.)
WB (Western Blot)
(Western blot analysis of ESRRA (arrow) using rabbit polyclonal ESRRA Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ESRRA gene.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 293 cells using ESRRA Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 293 cells using ESRRA Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with ESRRA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with ESRRA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of ESRRA Antibody (Center) in A375 cell line lysates (35ug/lane). ESRRA (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of ESRRA Antibody (Center) in A375 cell line lysates (35ug/lane). ESRRA (arrow) was detected using the purified Pab.)
Related Product Information for anti-ESRRA antibody
ESRRA is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes.
Product Categories/Family for anti-ESRRA antibody
References
Yang,N., et.al., J. Biol. Chem. 271 (10), 5795-5804 (1996)
Wiley,S.R., et.al., Genes Dev. 7 (11), 2206-2219 (1993)
Wiley,S.R., et.al., Genes Dev. 7 (11), 2206-2219 (1993)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45510
NCBI Official Full Name
steroid hormone receptor ERR1 isoform 1
NCBI Official Synonym Full Names
estrogen-related receptor alpha
NCBI Official Symbol
ESRRA
NCBI Official Synonym Symbols
ERR1; ERRa; ESRL1; NR3B1; ERRalpha
NCBI Protein Information
steroid hormone receptor ERR1
UniProt Protein Name
Steroid hormone receptor ERR1
UniProt Gene Name
ESRRA
UniProt Synonym Gene Names
ERR1; ESRL1; NR3B1; ERR-alpha
UniProt Entry Name
ERR1_HUMAN
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Product Notes
The ESRRA esrra (Catalog #AAA289083) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 131-159. The ESRRA Antibody (Center) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ESRRA can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ESRRA esrra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ESRRA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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