IHC (Immunohistochemisry)
(Dilution: Tissue/cell: rat uterus tissue ; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-ETL Polyclonal Antibody, Unconjugated(bs-13111R) 1:500, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
Rabbit anti-Rat ETL Polyclonal Antibody | anti-ETL antibody
ETL Polyclonal Antibody
IHC (Immunohistochemisry)
(Dilution: Tissue/cell: rat uterus tissue ; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-ETL Polyclonal Antibody, Unconjugated(bs-13111R) 1:500, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
IHC (Immunohistochemisry)
(Dilution: Tissue/cell: rat uterus tissue ; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-ETL Polyclonal Antibody, Unconjugated(bs-13111R) 1:500, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
IF (Immunofluorescence)
(Tissue/cell: rat uterus tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-ETL Polyclonal Antibody, Unconjugated(bs-13111R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)
IF (Immunofluorescence)
(Tissue/cell: rat uterus tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-ETL Polyclonal Antibody, Unconjugated(bs-13111R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)
IF (Immunofluorescence)
(Tissue/cell: rat uterus tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-ETL Polyclonal Antibody, Unconjugated(bs-13111R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)
IF (Immunofluorescence)
(Tissue/cell: rat uterus tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-ETL Polyclonal Antibody, Unconjugated(bs-13111R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)
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Product Notes
The ETL eltd1 (Catalog #AAA291448) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ETL Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ETL can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ETL eltd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ETL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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