WB (Western Blot)
(Detection of human and mouse EWS by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody AAA210940 (lot AAA210940-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Rabbit anti-Human, Mouse EWS Polyclonal Antibody | anti-EWS antibody
Rabbit anti-EWS Antibody, Affinity Purified
Gene Names
EWSR1; EWS; bK984G1.4
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
EWS, Antibody; Rabbit anti-EWS Antibody, Affinity Purified; EWS RNA-binding protein variant 6; bK984G1.4; Ewing sarcoma breakpoint region 1; Ewing sarcoma breakpoint region 1 protein; Ewings sarcoma EWS-Fli1 (type 1) oncogene; EWS; EWS-FLI1; RNA-binding protein EWS; EWS oncogene; EWS RNA binding protein 1; anti-EWS antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-EWS antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 125 and 175
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse EWS by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody AAA210940 (lot AAA210940-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
WB (Western Blot)
(Detection of human and mouse EWS by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody AAA210940 (lot AAA210940-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human EWS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EWS antibody AAA210940 (lot AAA210940-2) used for IP at 3 ug per reaction. EWS was also immunoprecipitated by a previous lot of this antibody (lot AAA210940-1). For blotting immunoprecipitated EWS, AAA210940 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human EWS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EWS antibody AAA210940 (lot AAA210940-2) used for IP at 3 ug per reaction. EWS was also immunoprecipitated by a previous lot of this antibody (lot AAA210940-1). For blotting immunoprecipitated EWS, AAA210940 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human EWS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EWS (Cat. No. AAA210940 Lot2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human EWS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EWS (Cat. No. AAA210940 Lot2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-EWS antibody
EWS is a member of a family of RNA-binding proteins that may serve as adapters that bridge RNA polymerase II complex and RNA splicing factors. Translocations and fusions between EWS and the ETS transcription factors promote oncogenesis and are associated with Ewing's family tumors.
Product Categories/Family for anti-EWS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,508 Da
NCBI Official Full Name
RNA-binding protein EWS isoform 2
NCBI Official Synonym Full Names
EWS RNA-binding protein 1
NCBI Official Symbol
EWSR1
NCBI Official Synonym Symbols
EWS; bK984G1.4
NCBI Protein Information
RNA-binding protein EWS; EWS RNA-binding protein variant 6; Ewing sarcoma breakpoint region 1; Ewings sarcoma EWS-Fli1 (type 1) oncogene
UniProt Protein Name
RNA-binding protein EWS
UniProt Gene Name
EWSR1
UniProt Synonym Gene Names
EWS
UniProt Entry Name
EWS_HUMAN
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Product Notes
The EWS ewsr1 (Catalog #AAA210940) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-EWS Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EWS can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the EWS ewsr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EWS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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