FCM/FACS (Flow Cytometry)
(EXOSC8 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human EXOSC8 Polyclonal Antibody | anti-EXOSC8 antibody
EXOSC8 Antibody (C-term)
Gene Names
EXOSC8; p9; CIP3; EAP2; OIP2; PCH1C; RRP43; Rrp43p; bA421P11.3
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
EXOSC8, Antibody; EXOSC8 Antibody (C-term); Exosome complex component RRP43; Exosome component 8; Opa-interacting protein 2; OIP-2; Ribosomal RNA-processing protein 43; p9; EXOSC8; OIP2; RRP43; anti-EXOSC8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EXOSC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-276 amino acids from the C-terminal region of human EXOSC8.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
243-276
Sequence Length
276
Applicable Applications for anti-EXOSC8 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(EXOSC8 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(EXOSC8 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue with EXOSC8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue with EXOSC8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of anti-EXOSC8 Antibody (C-term) in HL60 cell line lysates (35ug/lane). EXOSC8(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-EXOSC8 Antibody (C-term) in HL60 cell line lysates (35ug/lane). EXOSC8(arrow) was detected using the purified Pab.)
Related Product Information for anti-EXOSC8 antibody
EXOSC8 is a component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3'-untranslated regions. It is required for the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA and has a 3'-5' exonuclease activity.
References
Raijmakers,R., J. Mol. Biol. 323 (4), 653-663 (2002)
Jiang,T., Proc. Natl. Acad. Sci. U.S.A. 99 (8), 5295-5300 (2002)
Raijmakers,R., J. Mol. Biol. 315 (4), 809-818 (2002)
Brouwer,R., J. Biol. Chem. 276 (9), 6177-6184 (2001)
Jiang,T., Proc. Natl. Acad. Sci. U.S.A. 99 (8), 5295-5300 (2002)
Raijmakers,R., J. Mol. Biol. 315 (4), 809-818 (2002)
Brouwer,R., J. Biol. Chem. 276 (9), 6177-6184 (2001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30040
NCBI Official Full Name
exosome complex component RRP43
NCBI Official Synonym Full Names
exosome component 8
NCBI Official Symbol
EXOSC8
NCBI Official Synonym Symbols
p9; CIP3; EAP2; OIP2; PCH1C; RRP43; Rrp43p; bA421P11.3
NCBI Protein Information
exosome complex component RRP43
UniProt Protein Name
Exosome complex component RRP43
UniProt Gene Name
EXOSC8
UniProt Synonym Gene Names
OIP2; RRP43; OIP-2
UniProt Entry Name
EXOS8_HUMAN
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Product Notes
The EXOSC8 exosc8 (Catalog #AAA285387) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 243-276. The EXOSC8 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EXOSC8 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the EXOSC8 exosc8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EXOSC8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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