Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: XPo1 knockout Hela cell lysate; Predicted MW: 123kd Observed MW: 110kd Primary Ab: 1ug/ml Rabbit Anti-Human XPO1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Rabbit anti-Human, Pig Exportin 1 Polyclonal Antibody | anti-XPO1 antibody
Exportin 1 (XPO1) Polyclonal Antibody
Gene Names
XPO1; emb; CRM1; exp1
Reactivity
Human, Pig
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Exportin 1, Antibody; Exportin 1 (XPO1) Polyclonal Antibody; Exp1; CRM1 Homolog, Yeast; Chromosome region maintenance 1 protein homolog; anti-XPO1 antibody
Host
Rabbit
Reactivity
Human, Pig
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against XPO1. It has been selected for its ability to recognize XPO1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-XPO1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human, Pig
Organism Species
Human
Fragment
XPo1 (Thr917~Asp1071)
Quality Control
Content: The quality control contains recombinant XPo1 (Thr917~Asp1071) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: XPo1 knockout Hela cell lysate; Predicted MW: 123kd Observed MW: 110kd Primary Ab: 1ug/ml Rabbit Anti-Human XPO1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: XPo1 knockout Hela cell lysate; Predicted MW: 123kd Observed MW: 110kd Primary Ab: 1ug/ml Rabbit Anti-Human XPO1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human K562 Cells; Lane3: Human Lung Tissue; Lane4: Porcine Intestine Tissue.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human K562 Cells; Lane3: Human Lung Tissue; Lane4: Porcine Intestine Tissue.)
Product Categories/Family for anti-XPO1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
123,386 Da
NCBI Official Full Name
exportin-1
NCBI Official Synonym Full Names
exportin 1
NCBI Official Symbol
XPO1
NCBI Official Synonym Symbols
emb; CRM1; exp1
NCBI Protein Information
exportin-1
UniProt Protein Name
Exportin-1
UniProt Gene Name
XPO1
UniProt Synonym Gene Names
CRM1; Exp1
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Product Notes
The XPO1 xpo1 (Catalog #AAA21017) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Exportin 1 (XPO1) Polyclonal Antibody reacts with Human, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Exportin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the XPO1 xpo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Exportin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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