WB (Western Blot)
(Western blot analysis of extracts from Mouse kidney, using EYA3 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit EYA3 Polyclonal Antibody | anti-EYA3 antibody
EYA3 Antibody
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
EYA3, Antibody; EYA3 Antibody; AI844637; DKFZp686C132; EYA3; EYA3_HUMAN; Eyes absent 3; Eyes absent 3 homolog (Drosophila); Eyes absent homolog 3; OTTMUSP00000010370; RP11-460I13.4; RP23-442A20.1; anti-EYA3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
EYA3 Antibody detects endogenous levels of EYA3.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
573
Applicable Applications for anti-EYA3 antibody
WB (Western Blot)
Immunogen
A synthesized peptide derived from human EYA3.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from Mouse kidney, using EYA3 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Mouse kidney, using EYA3 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells, using EYA3 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells, using EYA3 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-EYA3 antibody
Description: Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1 (PubMed:19234442, PubMed:19351884). Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity (By similarity). May be involved in development of the eye.
Post Translational Modifications: Ser-266 phosphorylation is required for localization at sites of DNA damage and directing interaction with H2AX.
Subunit Structure: Interacts with SIX1 and DACH1, and probably SIX2, SIX4, SIX5.
Similarity: Belongs to the HAD-like hydrolase superfamily. EYA family.
Post Translational Modifications: Ser-266 phosphorylation is required for localization at sites of DNA damage and directing interaction with H2AX.
Subunit Structure: Interacts with SIX1 and DACH1, and probably SIX2, SIX4, SIX5.
Similarity: Belongs to the HAD-like hydrolase superfamily. EYA family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 66 kDa
Predicted: 63 kDa
Predicted: 63 kDa
NCBI Official Full Name
EYA3
NCBI Official Synonym Full Names
EYA transcriptional coactivator and phosphatase 3
NCBI Official Symbol
EYA3
NCBI Protein Information
eyes absent homolog 3
UniProt Protein Name
Eyes absent homolog 3
UniProt Gene Name
EYA3
UniProt Entry Name
EYA3_HUMAN
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Product Notes
The EYA3 eya3 (Catalog #AAA328850) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EYA3 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's EYA3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the EYA3 eya3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EYA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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