WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-EZH2(Thr367) Ab.Lane 1: Rat brain lysates;Lane 2: Mouse brain lysates;Lane 3: Rat muscle lysates;Lane 4: Rat muscle lysates treated with blocking peptide;)
Rabbit EZH2 Polyclonal Antibody | anti-EZH2 antibody
Phospho-EZH2 (Thr367) Antibody
Gene Names
EZH2; WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
EZH2, Antibody; Phospho-EZH2 (Thr367) Antibody; Enhancer of zeste 2; enhancer of zeste 2 polycomb repressive complex 2 subunit; Enhancer of zeste homolog 2 (Drosophila); Enhancer of zeste homolog 2; Enhancer of zeste, Drosophila, homolog 2; ENX 1; Enx 1h; ENX-1; ENX1; Enx1h; EZH 2; EZH1; EZH2; EZH2_HUMAN; EZH2b; Histone-lysine N-methyltransferase EZH2; KMT 6; KMT6; KMT6A; Lysine N-methyltransferase 6; MGC9169; WVS; WVS2; anti-EZH2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Supplied in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-EZH2 antibody
Western Blot (WB)
Application Notes
WB: 1:1000-3000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human EZH2 around the phosphorylation site of T367.
Preparation and Storage
Store at -20 degrees C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-EZH2(Thr367) Ab.Lane 1: Rat brain lysates;Lane 2: Mouse brain lysates;Lane 3: Rat muscle lysates;Lane 4: Rat muscle lysates treated with blocking peptide;)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-EZH2(Thr367) Ab.Lane 1: Rat brain lysates;Lane 2: Mouse brain lysates;Lane 3: Rat muscle lysates;Lane 4: Rat muscle lysates treated with blocking peptide;)
Related Product Information for anti-EZH2 antibody
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed:22323599). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85kD, 100kD
NCBI Official Full Name
histone-lysine N-methyltransferase EZH2 isoform c
NCBI Official Synonym Full Names
enhancer of zeste homolog 2 (Drosophila)
NCBI Official Symbol
EZH2
NCBI Official Synonym Symbols
WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
NCBI Protein Information
histone-lysine N-methyltransferase EZH2; lysine N-methyltransferase 6
UniProt Protein Name
Histone-lysine N-methyltransferase EZH2
UniProt Gene Name
EZH2
UniProt Synonym Gene Names
KMT6
UniProt Entry Name
EZH2_HUMAN
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Product Notes
The EZH2 ezh2 (Catalog #AAA31470) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-EZH2 (Thr367) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EZH2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000-3000 *The optimal dilutions should be determined by the end user. IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. . Researchers should empirically determine the suitability of the EZH2 ezh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EZH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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