anti-Human F11 / FXI / Factor XI Polyclonal Antibody | anti-F11 antibody

Anti-F11 / FXI / Factor XI Antibody IHC-plus

Cat. Num. AAA52654

• Gene Names: F11; FXI
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified
• Synonyms: F11 / FXI / Factor XI, Antibody; Anti-F11 / FXI / Factor XI Antibody IHC-plus; Sheep Polyclonal (IgG) to Human F11 / FXI / Factor XI; Human F11 / FXI / Factor XI; Coagulation factor XI; Factor xi deficiency; F11 deficiency; Factor XI; PTA deficiency; PTA; Rosenthal syndrome; FXI; anti-F11 antibody

• Host: F11/ FX1/Factor XI antibody was produced in Sheep.
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Factor XI.
• Purity/Purification: Purified
• Form/Format: ddH20, 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 625

• Applicable Applications for anti-F11 antibody: WB (Western Blot), ELISA
• Family/Subfamily: Protease/Serine S1
• Immunogen Species: F11/FX1/Factor XI antibody was raised against Human
• Immunogen: F11/FX1/Factor XI antibody was raised against purified human Factor XI.
• Note: During shipment,small volumes of profuct will occasionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide .The chemical,physical and toxicological properties of thsi material have not been thoroughly investigated. Standard Laboratory practices should be followed. Avoid skin and eye contact, inhalation and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plumbing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent acumulations.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20° C. Aliquots are stable for at least 12 months.

Related Product Information for anti-F11 antibody

F11 Antibody, Coagulation factor XI Antibody, Factor xi deficiency Antibody, F11 deficiency Antibody, Factor XI Antibody, PTA deficiency Antibody, PTA Antibody, Rosenthal syndrome Antibody, FXI Antibody Description: F11 / FXI / Factor XI is coagulation factor XI of the blood coagulation cascade. This protein is present in plasma as a zymogen, which is a unique plasma coagulation enzyme because it exists as a homodimer consisting of two identical polypeptide chains linked by disulfide bonds.

NCBI and Uniprot Product Information

• NCBI GI #: 4503627
• NCBI GeneID: 2160
• NCBI Accession #: NP_000119.1
• NCBI GenBank Nucleotide #: NM_000128.3
• UniProt Accession #: P03951; Q4W5C2; Q9Y495; D3DP64
• NCBI Official Full Name: coagulation factor XI preproprotein
• NCBI Official Synonym Full Names: coagulation factor XI
• NCBI Official Symbol: F11
• NCBI Official Synonym Symbols: FXI
• NCBI Protein Information: coagulation factor XI
• UniProt Protein Name: Coagulation factor XI
• UniProt Gene Name: F11
• UniProt Synonym Gene Names: FXI; PTA
• UniProt Entry Name: FA11_HUMAN